Anti-STK36 Rabbit Polyclonal Antibody for WB, ELISA - Q9NRP7

Core Product Specifications and Parameters

Biological Background: Serine/threonine-protein kinase 36 (STK36) Function and Localization

• STK36 (serine/threonine-protein kinase 36), also known as Fused homolog, is a protein kinase encoded by the STK36 gene. Alternative splicing produces multiple isoforms.
• Plays a critical role in the Hedgehog signaling pathway by regulating GLI transcription factors. STK36 opposes the negative regulator SUFU and promotes nuclear localization of GLI1, GLI2, and GLI3. Related references: PMID:10806483, PMID:31279575, PMID:38096226
• STK36 directly phosphorylates GLI proteins, facilitating their dissociation from SUFU, a key step for transcriptional activation. Related references: PMID:28543983, PMID:31279575, PMID:38096226
• GLI2 requires an additional function of STK36 beyond its kinase activity to become transcriptionally active. Related references: PMID:10806483
• Localizes to the cilium, axonemal cytoskeleton, cytoplasm, and nucleus, consistent with its roles in signaling and ciliary function.
• Highly expressed in adult testis germ cells and respiratory epithelial cells; low expression in most fetal tissues and adult ovaries. Related references: PMID:10806483, PMID:28543983
• Essential for postnatal development, construction of the central pair apparatus of motile cilia, and implicated in primary ciliary dyskinesia (CILD46). Related references: PMID:28543983

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 250-450 of human STK36 (NP_056505.2); the antibody may detect multiple isoforms due to alternative splicing, so validate in your sample system.
• For Western blot, a band around 144 kDa is expected; consider that post-translational modifications or isoforms may produce additional bands.
• Cross-reactivity with mouse is indicated, making this antibody suitable for murine model studies; verify performance under your experimental conditions.
• Optimize antibody dilution for WB and ELISA; recommended starting dilutions should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For researchers investigating the smoothened pathway, ciliopathies, or GLI transcription factors, we provide reliable sourcing of anti-STK36 antibodies and related targets. Our one-stop procurement services support diagnostic manufacturers and laboratories from concept to clinical application.