Anti-STK33 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q9BYT3

Core Product Specifications and Parameters

Biological Background: STK33 Function and Localization

• Encodes a serine/threonine protein kinase essential for spermatid differentiation and male fertility, as demonstrated by functional studies. Related references: PMID:37146716 PMID:38781365
• Promotes sperm flagella assembly during spermatogenesis by mediating phosphorylation of fibrous sheath proteins AKAP3 and AKAP4.
• Phosphorylates vimentin/VIM, regulating intermediate filament cytoskeleton dynamics.
• Localizes predominantly in the cytoplasm, with enrichment in the perinuclear region and cytoskeleton.
• Exhibits high expression in testis, fetal lung, and heart; moderate expression in pituitary gland, kidney, pancreas, and several other tissues; absent in nervous system tissues.
• Keywords: 3D-structure, Alternative splicing, ATP-binding, Cytoplasm, Cytoskeleton, Differentiation, Kinase, Nucleotide-binding, Phosphoprotein, Serine/threonine-protein kinase, Spermatogenesis, Transferase.

Experimental Guidance and Technical Tips

• The immunogen (aa 399-514) corresponds to the C-terminal region; consider its potential to detect full-length STK33 and any alternative isoforms.
• For Western blot (WB), a band around 58 kDa is expected; verify specificity by including positive (e.g., testis lysate) and negative controls.
• IF/ICC applications may require optimization of fixation and permeabilization conditions due to the cytoplasmic and cytoskeletal localization.
• ELISA performance may vary with antigen presentation; consider titration of antibody and antigen for optimal signal-to-noise ratio.
• Cross-reactivity with mouse has been confirmed; validate in the relevant sample system for other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to providing diagnostic manufacturers with reliable, one-stop access to IVD raw materials, supporting your projects from concept to clinic. This STK33 antibody, focused on kinase signaling and male fertility research, exemplifies our capability to supply validated monoclonal antibodies alongside complementary reagents such as antibody pairs, ancillary proteins, and bulk formulations. Contact our team to discuss customization, scale-up, or sourcing of rare targets for your diagnostic development needs.