Anti-STK19 Rabbit Polyclonal Antibody for WB, ELISA - P49842

Core Product Specifications and Parameters

Biological Background: STK19/WHR1 Function and Localization

• STK19/WHR1 is a tandem winged helix DNA-binding protein, originally annotated as an inactive serine/threonine-protein kinase (aliases: G11, RP1, D6S60, HLA-RP1).
• Functions as an essential component of the transcription-coupled nucleotide excision repair (TC-NER) complex, assembling at stalled RNA polymerase II at DNA lesions to ensure efficient repair. Related references: PMID:38252411, PMID:38890355, PMID:39353615, PMID:39547223, PMID:39547228, PMID:39547229
• Directly interacts with RNAPII and downstream DNA, and promotes the removal of stalled RNAPII to allow access for downstream repair factors. Related references: PMID:39547228, PMID:39547229
• Interacts with the XPD helicase subunit of TFIIH, guiding TFIIH to the DNA downstream of stalled RNAPII for repair. Related references: PMID:39353615, PMID:39547228, PMID:39547229
• Required for UVSSA monoubiquitination and regulates its repositioning and stabilization within the TC-NER complex; also stimulates ubiquitination of RNAPII subunit RBP1. Related references: PMID:39353615, PMID:39547223
• Binds RNA and modulates the expression levels of many mRNAs, suggesting additional post-transcriptional functions. Related references: PMID:38252411, PMID:38890355
• Localizes to the nucleus and cytoplasm.
• Expressed in monocytes, hepatocytes, epithelial cells, and T- and B-lymphocytes.

Experimental Guidance and Technical Tips

• The immunogen (aa 75-364 of human STK19) covers the tandem winged helix domains, likely contributing to cross-reactivity with mouse and rat due to sequence conservation.
• For Western blotting, use whole-cell or nuclear lysates from human, mouse, or rat tissues/cell lines (e.g., monocytes, hepatocytes); the expected band is approximately 41 kDa, which may differ from theoretical predictions due to post-translational modifications or isoform variations.
• In ELISA applications, validate detection sensitivity with recombinant STK19 protein or characterized positive control samples.
• Consider inducing TC-NER with UV irradiation or DNA-damaging agents to enrich chromatin-associated STK19 for more robust detection, especially in nuclear fractions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies a comprehensive range of IVD raw materials, including this anti-STK19 polyclonal antibody, to support DNA repair and genomic stability research. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing services to accelerate your diagnostic development. Partner with CamelBio to streamline procurement from concept to clinic.