Anti-STIP1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P31948

Core Product Specifications and Parameters

Biological Background: STIP1 Function and Localization

• STIP1 (Stress-induced-phosphoprotein 1), also known as Hop (Hsc70/Hsp90-organizing protein), is a 62.6 kDa co-chaperone encoded by the STIP1 gene in humans.
• It acts as a co-chaperone for HSP90AA1, mediating the association of the molecular chaperones HSPA8/HSC70 and HSP90 to facilitate protein folding and maturation.
  Related references:
  PMID:27353360
• Alternative names include renal carcinoma antigen NY-REN-11 and transformation-sensitive protein IEF SSP 3521.
• Localizes mainly to the cytoplasm, but also found in the nucleus and dynein axonemal particles.
• Undergoes various post-translational modifications including acetylation, phosphorylation, and ubiquitination, with multiple TPR repeat domains contributing to its chaperone interactions.
• Keywords associated with STIP1 include: 3D-structure, Acetylation, Alternative splicing, Cytoplasm, Direct protein sequencing, Isopeptide bond, Nucleus, Phosphoprotein, Proteomics identification, Reference proteome, Repeat, TPR repeat, Ubl conjugation.
• It may have roles in stress response, as indicated by its transformation-sensitive and stress-induced nature.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 2-98 of human STIP1, an N-terminal region conserved across species, supporting its broad cross-reactivity; validate with your target species if needed.
• For WB, consider testing a dilution series (e.g., 1:500–1:5000) based on the antibody concentration and the endogenous expression levels in your sample.
• IHC-P may require optimization of antigen retrieval and blocking conditions; include appropriate positive and negative controls.
• Consistent detection in human, mouse, and rat samples suggests suitability for translational research studies; confirm performance in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with a comprehensive one-stop platform for IVD raw materials, technical support, and sourcing consulting. This STIP1 antibody supports investigations into chaperone biology, stress signaling, and oncology. From validated antibody pairs to bulk ancillary reagents, we streamline procurement so you can focus on accelerating your project from concept to clinic.