Anti-STIP1 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P31948

Core Product Specifications and Parameters

Biological Background: STIP1 Function and Localization

• Stress-induced-phosphoprotein 1 (STIP1), also known as Hsc70/Hsp90-organizing protein (Hop), renal carcinoma antigen NY-REN-11, or transformation-sensitive protein IEF SSP 3521, is a 63 kDa co-chaperone encoded by the STIP1 gene.
• Acts as a co-chaperone for HSP90AA1 and mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90. Related references: PMID:27353360
• The protein shuttles between the cytoplasm and nucleus, and is also found in dynein axonemal particles, reflecting its dynamic role in chaperone complexes.
• STIP1 contains multiple tetratricopeptide repeat (TPR) domains that facilitate protein–protein interactions with heat shock proteins.
• Post-translational modifications include phosphorylation and acetylation, which may regulate its co-chaperone activity and subcellular distribution.
• Referenced in proteomics identification and alternative splicing databases, with evidence at protein level, supporting its broad physiological relevance.

Experimental Guidance and Technical Tips

• The immunogen was designed against amino acids 1–300 of human STIP1; consider this region when assessing epitope accessibility in fixed or native conformations.
• For WB, a band around 63 kDa is expected in lysates from human, mouse, rat, or monkey cells; verify using appropriate positive controls.
• IHC-P and IF/ICC may require antigen retrieval or permeabilization optimization; validate staining patterns against known subcellular localizations (cytoplasmic/nuclear).
• In IP experiments, include appropriate IgG controls and consider the co-chaperone complex dynamics; co-precipitation of HSP90 or HSC70 may be observed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target acquisition. For stress biology and cancer research involving the STIP1 co-chaperone, our portfolio includes key reagents such as this anti-STIP1 antibody to support assay development and diagnostic manufacturing. We streamline procurement from concept to clinic, ensuring reliable supply for your workflow integration.