Anti-STIM2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9P246

Core Product Specifications and Parameters

Biological Background: STIM2 Function and Localization

• STIM2 is a highly sensitive Ca²⁺ sensor in the endoplasmic reticulum membrane that regulates both store-operated and store-independent calcium entry.
• It plays a key role in mediating store-operated Ca²⁺ entry (SOCE), a process triggered by depletion of intracellular stores, by translocating to the plasma membrane to activate CRAC channels ORAI1, ORAI2, and ORAI3.
• STIM2 may inhibit STIM1-mediated Ca²⁺ influx, suggesting a regulatory balance between these two stromal interaction molecules in calcium homeostasis.
• The protein is widely expressed across all tissues and tumor cell lines, indicating a ubiquitous role in cellular calcium signaling.
• Subcellularly, it localizes to the endoplasmic reticulum membrane and relocates to the plasma membrane upon ER Ca²⁺ store depletion.
• The STIM2 gene undergoes alternative splicing, producing isoforms that may contribute to functional diversity.
• Post-translational modifications include glycosylation and phosphorylation, which may regulate its activity.
• UniProt annotations also highlight the presence of coiled-coil domains and a signal peptide, consistent with its ER membrane insertion and protein interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 60-180 of human STIM2 (NP_001162588). This region is within the N-terminal ER-luminal domain, which is expected to be accessible in native membrane preparations, making this antibody suitable for applications such as Western blot and immunoprecipitation.
• For Western blot, the predicted molecular weight of STIM2 is approximately 84 kDa; however, glycosylation may cause a slight shift in migration. Include appropriate positive and negative controls.
• The antibody has been reported to detect STIM2 from human, mouse, and rat samples. Validate cross-reactivity in your specific model system before quantitative assays.
• For immunohistochemistry (IHC-P), consider antigen retrieval and test antibody dilutions as staining intensity may vary depending on tissue fixation and expression levels.
• In immunofluorescence/ICC, STIM2 is expected to localize to the endoplasmic reticulum under resting conditions and may translocate to the plasma membrane upon store depletion; co-staining with ER markers is recommended to confirm localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a one-stop sourcing solution for IVD raw materials. This anti-STIM2 polyclonal antibody is part of our portfolio targeting calcium signaling and store-operated calcium entry, critical for understanding cellular homeostasis. Beyond antibodies, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to streamline your assay development from concept to clinic.