Anti-STIM1 Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q13586

Core Product Specifications and Parameters

Biological Background: STIM1 Function and Localization

• Acts as a Ca2+ sensor that gates two major inward rectifying Ca2+ channels at the plasma membrane: Ca2+ release-activated Ca2+ (CRAC) channels and arachidonate-regulated Ca2+-selective (ARC) channels. Related references: PMID:15866891, PMID:16005298, PMID:16208375
• Plays a key role in store-operated Ca2+ entry (SOCE). Upon Ca2+ store depletion, STIM1 translocates from the endoplasmic reticulum to the plasma membrane and activates ORAI1, ORAI2, and ORAI3 subunits to induce sustained and oscillatory Ca2+ influx. Related references: PMID:16208375, PMID:16537481, PMID:32415068
• Involved in enamel formation. Related references: PMID:24621671
• Regulates the type I interferon response by retaining the signaling adapter STING at the endoplasmic reticulum. Related references: PMID:30643259
• Subcellular localization: cell membrane, endoplasmic reticulum membrane, cytoplasm, cytoskeleton, and sarcoplasmic reticulum.
• Ubiquitously expressed in various human primary cells and tumor cell lines.
• Post-translational modifications include glycosylation and phosphorylation; alternative splicing yields multiple isoforms.
• Contains coiled-coil domains and transmembrane helices; molecular weight ~77 kDa (calculated 77,423 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal cytoplasmic domain (aa 451–685), making this antibody suitable for detecting both endogenous and overexpressed STIM1.
• For Western blot, a band near 77 kDa is expected; validate detection in your sample system, as isoform-specific patterns may occur.
• For immunohistochemistry (IHC-P), consider antigen retrieval protocols to unmask epitopes in formalin-fixed paraffin-embedded tissues.
• For immunoprecipitation (IP), optimize antibody concentration and incubation conditions for efficient capture of STIM1 complexes.
• For ELISA, the antibody can be used as a capture or detection reagent; confirm linearity and sensitivity with recombinant protein standards.
• As a polyclonal antibody, performance may vary between production batches; include appropriate controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including this anti-STIM1 polyclonal antibody, to diagnostic manufacturers and researchers. With validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, we streamline your procurement from discovery to clinical application. Contact us for rare target sourcing and tailored support in calcium signaling research.