Anti-STIM1 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q13586

Core Product Specifications and Parameters

Biological Background: STIM1 Function and Localization

• STIM1 (stromal interaction molecule 1) is a 77 kDa transmembrane protein encoded by the human gene STIM1 (Gene ID: 6786). It belongs to the stromal interaction molecule family and is also known by aliases GOK, TAM, and TAM1.
• STIM1 functions as a Ca2+ sensor in the endoplasmic reticulum (ER) that gates store-operated Ca2+ entry (SOCE) by activating Ca2+ release-activated Ca2+ (CRAC) channels and arachidonate-regulated Ca2+-selective (ARC) channels at the plasma membrane (PubMed:15866891, PubMed:16005298, PubMed:16208375).
  Related references:
  PMID:15866891
  PMID:16005298
  PMID:16208375
• Upon store depletion, STIM1 oligomerizes and translocates from the ER to ER–plasma membrane junctions, where it directly binds and activates the ORAI family of CRAC channel subunits (ORAI1, ORAI2, ORAI3) to mediate sustained and oscillatory Ca2+ influx (PubMed:16208375, PubMed:16537481, PubMed:32415068).
  Related references:
  PMID:16208375
  PMID:16537481
  PMID:32415068
• Beyond calcium signaling, STIM1 is involved in enamel formation (PubMed:24621671) and regulates the type I interferon response by retaining the adaptor STING at the ER (PubMed:30643259).
  Related references:
  PMID:24621671
  PMID:30643259
• STIM1 is ubiquitously expressed in human primary cells and tumor cell lines, with subcellular localization at the ER membrane, cell membrane, cytoplasm, cytoskeleton, and sarcoplasmic reticulum.
• It undergoes post-translational modifications, including phosphorylation and glycosylation, and is featured in keywords such as phosphoprotein, glycoprotein, and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the cytoplasmic C-terminal region (amino acids 530–685) of human STIM1, which is critical for oligomerization and ORAI binding. This design may be particularly useful for detecting both monomeric and oligomeric STIM1 in WB and IP.
• For IF/ICC, consider co-staining with ER markers (e.g., calnexin) or plasma membrane markers to evaluate stimulus-dependent translocation. Validate in your specific cell type and treatment conditions.
• The antibody cross-reacts with mouse and rat STIM1, making it suitable for commonly used model systems. Titrate the antibody for your particular assay to optimize signal-to-noise ratio.
• Since STIM1 is a low-abundance signaling protein, appropriate blocking and washing conditions are essential to reduce non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a reliable one-stop provider for IVD raw materials, offering validated antibodies and recombinant proteins to support calcium signaling research. Our comprehensive portfolio includes optimized polyclonal and monoclonal antibodies like STIM1 Rabbit pAb, paired antibody sets, and bulk ancillary reagents, simplifying procurement for diagnostic manufacturers and research laboratories. Additionally, we offer rare target sourcing and technical consulting to accelerate your assay development from concept to clinic.