Anti-Sterol Carrier Protein 2 (SCP2) Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P22307

Core Product Specifications and Parameters

Biological Background: Sterol Carrier Protein 2 Function and Localization

• Plays a crucial role in the peroxisomal oxidation of branched-chain fatty acids, catalyzing the last step of beta‑oxidation of branched chain fatty acids and the side chain of bile acid intermediates di- and trihydroxycoprostanic acids (DHCA and THCA). Also active with medium and long straight chain 3‑oxoacyl‑CoAs. Related references: PMID:10706581
• Mediates the transfer of common phospholipids, cholesterol, and gangliosides from the endoplasmic reticulum to the plasma membrane, thereby contributing to intracellular lipid trafficking and potentially regulating steroidogenesis. Related references: PMID:17157249 PMID:7642518 PMID:8300590
• Stimulates the microsomal conversion of 7‑dehydrocholesterol to cholesterol and transfers phosphatidylcholine between membranes in vitro.
• Binds fatty acids and fatty acyl‑CoA, such as phytanoyl‑CoA, and may regulate phospholipid synthesis by enhancing exogenous fatty acid incorporation into glycerides.
• Isoforms SCP2 and SCPx cooperate in the peroxisomal oxidation of naturally occurring tetramethyl‑branched fatty acyl‑CoAs.
• Localizes to multiple subcellular compartments: peroxisome, cytoplasm, mitochondrion, and endoplasmic reticulum.
• Expressed in liver, fibroblasts, and placenta.
• Belongs to the thiolase family and possesses acyltransferase activity; annotated with keywords: lipid transport, lipid metabolism, peroxisome, transferase, phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1‑100 of human SCP2; this region is conserved across human, mouse, and rat, supporting the observed cross‑reactivity.
• For WB, an expected band is ~59 kDa (full‑length SCP2); consider using fresh or protease‑inhibited lysates to preserve peroxisomal proteins.
• In IHC‑P and IF/ICC, note the multitopic subcellular distribution (peroxisomes, mitochondria, cytoplasm, ER); validate staining patterns with appropriate organelle markers.
• For ELISA, the recombinant monoclonal format provides consistent lot‑to‑lot performance; optimize coating and detection conditions as needed.
• Cross‑reactivity with mouse and rat has been confirmed; still, verify in the relevant sample system before running large‑scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research groups by providing a one‑stop channel for IVD raw materials, including validated monoclonal and polyclonal antibodies like this anti‑SCP2 clone. Our portfolio covers lipid metabolism and peroxisomal targets, and we can source antibody pairs, ancillary reagents, and rare‑target raw materials in bulk to streamline your assay development. Whether you need consultation or tailored supply solutions, CamelBio bridges the gap from concept to clinic.