Anti-Steroidogenic factor-1 (SF-1/NR5A1) Rabbit Polyclonal Antibody for WB & ELISA - Q13285

Core Product Specifications and Parameters

Biological Background: Steroidogenic factor-1 (SF-1/NR5A1) Function and Localization

• Steroidogenic factor-1 (SF-1), encoded by the NR5A1 gene, is a nuclear receptor and transcriptional activator essential for sexual differentiation and the formation of primary steroidogenic tissues. Related references: PMID:27378692
• It binds to the Ad4 site in the promoter regions of key steroidogenic P450 genes (CYP11A, CYP11B, CYP21B) and also regulates AMH/Müllerian inhibiting substance, AHCH, and STAR gene expression. Related references: PMID:27378692
• SF-1 contains a zinc-finger DNA-binding domain and recognizes consensus sequences 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3'. Related references: PMID:27378692
• The protein binds phospholipids, including phosphatidylcholine and phosphoinositides, and its activity is enhanced by HIPK3-mediated phosphorylation upon cAMP signaling.
• Subcellular localization is predominantly nuclear, consistent with its role as a transcription factor.
• High expression is observed in the adrenal cortex, ovary, testis, and spleen, reflecting its critical role in steroidogenesis and sexual development.
• Post-translational modifications such as acetylation, phosphorylation, and ubiquitin-like conjugation modulate SF-1 activity, and mutations are linked to diseases like premature ovarian failure.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal 120 amino acids; the antibody may detect full-length SF-1 as well as N-terminally truncated isoforms. Validate in your sample system.
• For Western blot, optimize the primary antibody dilution using a positive control lysate from tissues with high SF-1 expression (e.g., adrenal or gonadal tissues).
• In ELISA, consider direct coating with the recombinant immunogen or capture of native SF-1 from cell lysates; perform checkerboard titrations.
• Cross-reactivity with mouse and rat has been reported; confirm specificity by using knockout controls or siRNA-mediated knockdown in your experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, from validated antibody pairs to bulk ancillary reagents tailored to steroidogenesis and reproductive biology research. In addition to this SF-1 antibody, we offer optimized monoclonal/polyclonal antibodies and can source rare-target raw materials, supporting your diagnostic development from concept to clinic.