Anti-Steroidogenic factor-1 (SF-1/NR5A1) Rabbit Monoclonal Antibody for WB, ELISA - Q13285

Core Product Specifications and Parameters

Biological Background: Steroidogenic factor-1 Function and Localization

• Steroidogenic factor-1 (SF-1/NR5A1) is a transcriptional activator essential for sexual differentiation and the formation of primary steroidogenic tissues, including the adrenal cortex and gonads. Related references: PMID:27378692
• It binds to the Ad4 site in the promoter regions of key steroidogenic P450 genes such as CYP11A, CYP11B, and CYP21B, as well as regulating AMH, AHCH, and STAR genes.
• SF-1 is a member of the nuclear receptor subfamily 5 group A (NR5A), also known as adrenal 4-binding protein (AD4BP) or steroid hormone receptor Ad4BP.
• Localized predominantly in the nucleus, SF-1 functions as a DNA-binding transcription factor.
• Post-translational modifications include phosphorylation by HIPK3, which enhances steroidogenic gene expression upon cAMP pathway stimulation.
• It can bind phospholipids, including phosphatidylcholine and phosphatidylinositol phosphates, which may modulate its activity.
• SF-1 is highly expressed in the adrenal cortex, ovary, testis, and spleen. Related references: PMID:9177385
• Additional keywords: zinc-finger, lipid-binding, disease variants linked to premature ovarian failure and disorders of sexual development.

Experimental Guidance and Technical Tips

• Based on the immunogen, the antibody epitope is mapped to the C-terminal region (amino acids 351-461) of human SF-1, which shares homology with mouse, supporting observed cross-reactivity.
• For Western blot, consider a band near 52kDa. Validate loading controls and run appropriate molecular weight markers.
• In ELISA, this monoclonal antibody may serve as a capture or detection reagent; however, assay optimization including antibody pairing and blocking conditions is recommended.
• Verify antibody specificity in the relevant sample system (e.g., adrenal cortex or gonadal cell lines) to account for tissue-specific expression and potential isoforms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories one-stop access to IVD raw materials, technical services, and consulting for every stage from concept to clinic. This anti-SF-1/NR5A1 antibody is suitable for reproductive endocrinology and steroidogenic disorder research; we also supply validated antibody pairs, recombinant proteins, and bulk ancillary reagents. For rare target or customized raw-material sourcing, our team provides responsive support to help bridge your assay development needs.