Anti-STEAP1 Rabbit Polyclonal Antibody for WB, ELISA - Q9UHE8

Core Product Specifications and Parameters

Biological Background: STEAP1 Function and Localization

• STEAP1 (Six-transmembrane epithelial antigen of prostate 1, also known as PRSS24 or STEAP) is a 40 kDa transmembrane protein belonging to the STEAP family.
• It localizes primarily to the endosome membrane and cell membrane, as indicated by subcellular localization data.
• STEAP1 does not function as a metalloreductase by itself due to the absence of an NADPH-binding domain; however, it can promote Fe³⁺ reduction when fused to the NADPH-binding domain of STEAP4.
• The protein contains FAD and heme cofactors, suggesting a role in electron transfer reactions.
• STEAP1 is ubiquitously expressed in normal tissues and is highly up-regulated in prostate tumors, making it a potential biomarker for prostate cancer.
• Keywords: 3D-structure, Cell membrane, Endosome, FAD, Flavoprotein, Heme, Iron, Metal-binding, Transmembrane.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein covering the N-terminal region (amino acids 1–80) of human STEAP1. This region may be exposed in native conformation, supporting use in ELISA.
• Expected band size in Western blot is approximately 40 kDa under reducing conditions; run appropriate molecular weight markers to confirm.
• Cross-reactivity with mouse and rat STEAP1 is predicted based on sequence conservation; validate in the relevant species tissue or cell lysates.
• For ELISA, consider using the antibody as capture or detection reagent; pairing with another clone may improve sensitivity.
• Always perform appropriate negative controls (e.g., knockout cell lines or peptide blocking) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive supply partner for IVD raw materials, offering a broad portfolio of validated antibodies, antigens, and ancillary reagents. This STEAP1 polyclonal antibody is suitable for prostate cancer and iron metabolism research applications; to accelerate your assay development, we can source matched antibody pairs, bulk quantities of key reagents, and even rare-target raw materials. With expert technical support from concept to clinic, CamelBio is committed to helping diagnostic manufacturers and laboratories streamline their sourcing process.