Anti-STC1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P52823

Core Product Specifications and Parameters

Biological Background: STC1 Function and Localization

• STC1 (Stanniocalcin-1, also known as STC) is a secreted glycoprotein hormone encoded by the STC1 gene. Alternative splicing generates multiple isoforms, expanding its regulatory potential.
• The protein stimulates renal phosphate reabsorption, playing a key role in calcium and phosphate homeostasis. It may therefore prevent hypercalcemia.
• As a classical secretory hormone, STC1 is synthesized with a signal peptide and released into the extracellular space, where it acts on target tissues including the kidney.
• Tissue expression is broad, with highest levels in ovary, prostate, heart, kidney, and thyroid. In the kidney, expression is confined to the distal convoluted tubule and collecting tubule.
• STC1 contains conserved disulfide bonds and N-linked glycosylation sites, which are important for its stability and biological activity.
• It is classified as a hormone and glycoprotein, and has been identified in proteomics studies, underscoring its relevance in endocrine and renal research.

Experimental Guidance and Technical Tips

• The immunogen used spans the mature STC1 protein (amino acids 18-247), so the polyclonal antibody likely recognizes multiple linear and conformational epitopes within this region.
• For Western blotting, a band at approximately 28 kDa is expected. Consider using reducing and non-reducing conditions to assess potential disulfide-linked oligomers.
• In immunofluorescence/ICC, fixation and permeabilization may influence detection of this secreted protein; validate with appropriate controls (e.g., untreated vs. stimulated cells, or use of secretion inhibitors).
• This antibody has been used in ELISA; when developing a sandwich immunoassay, pair it with a capture antibody recognizing a distinct epitope.
• The reported cross-reactivity with mouse and rat makes it suitable for preclinical models; verify sequence homology in your region of interest to ensure target recognition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop IVD raw-material sourcing partner, connecting diagnostic manufacturers and research laboratories with high-quality reagents like this anti-STC1 antibody. Whether you require validated antibody pairs, bulk ancillary reagents, or customized sourcing for rare-target proteins in calcium/phosphate metabolism, our integrated supply chain supports your projects from early research to clinical application. Let CamelBio streamline your procurement process and accelerate your development pipeline.