Anti-STC1 Rabbit Polyclonal Antibody for WB and ELISA - P52823

Core Product Specifications and Parameters

Biological Background: STC1 Function and Localization

• STC1 (Stanniocalcin-1), also known as STC, is a secreted glycoprotein hormone involved in mineral homeostasis.
• Its primary function is to stimulate renal phosphate reabsorption, thereby potentially preventing hypercalcemia.
• STC1 is expressed in many tissues, with the highest levels in ovary, prostate, heart, kidney, and thyroid; within the kidney, it localizes to the distal convoluted tubule and collecting tubule.
• The protein is not detected in brain, liver, spleen, peripheral blood leukocytes, or adrenal medulla.
• As a secreted factor, STC1 contains a signal peptide and undergoes post-translational modifications including glycosylation and disulfide bond formation.
• Alternative splicing generates multiple isoforms, adding functional diversity.
• The theoretical molecular weight of the proprotein is 27.6 kDa; the mature glycoprotein typically migrates at approximately 28 kDa under reducing conditions.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a recombinant protein covering amino acids 18–247 of human STC1; it targets an internal region common to several isoforms.
• It is suitable for Western blot (WB) and ELISA. For WB, consider a starting dilution range of 1:500–1:2000 and validate in sample systems known to express STC1, such as kidney or ovary lysates.
• The antibody cross-reacts with mouse and rat STC1, enabling its use in comparative studies, though individual optimization is recommended for each species.
• Due to the polyclonal nature, batch-to-batch consistency should be verified if used in long-term projects.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutions with a comprehensive range of IVD raw materials, including validated antibody pairs and polyclonal antibodies like this anti-STC1 product. We support your projects in mineral metabolism and kidney function research, offering reliable sourcing for bulk ancillary reagents and rare-target raw materials. Our integrated services streamline your path from concept to clinic, ensuring you have the critical tools for success.