Anti-STAU2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NUL3

Core Product Specifications and Parameters

Biological Background: STAU2 Function and Localization

• STAU2 (Staufen homolog 2) is a double-stranded RNA-binding protein belonging to the Staufen family, characterized by conserved dsRNA-binding domains (dsRBDs).
• It plays a critical role in mRNA transport, mediating microtubule-dependent trafficking of select neuronal mRNAs from the cell body to dendrites.
• The localization of specific mRNAs to dendrites, and subsequent local protein synthesis, is essential for neurite outgrowth and synaptic plasticity.
• Subcellularly, STAU2 is found in the cytoplasm, nucleus, nucleolus, and endoplasmic reticulum, reflecting dynamic shuttling and involvement in multiple steps of RNA metabolism.
• Post-translational modifications include phosphorylation, as indicated by the Phosphoprotein keyword in UniProt.
• Additional annotations include alternative splicing, microtubule association, and designation as a reference proteome component.
• The canonical isoform has a calculated molecular weight of approximately 63 kDa (UniProt: 62608 Da), consistent with the observed band at 63 kDa.

Experimental Guidance and Technical Tips

• For Western blotting, use standard lysate preparation; expect a dominant band near 63 kDa. Validate specificity with appropriate positive and negative controls.
• In immunofluorescence/ICC, fixation with 4% PFA and permeabilization with 0.1% Triton X-100 is recommended to detect cytoplasmic, nuclear, or nucleolar pools of STAU2.
• For ELISA, consider using the immunogenic peptide or recombinant STAU2 protein as coating antigen, and perform checkerboard titrations to determine optimal antibody concentration.
• Cross-reactivity with mouse and rat is reported; confirm sequence homology in the immunogen region if applying to other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive raw-material solutions for diagnostic manufacturers and research laboratories, streamlining development from concept to clinic. This STAU2 polyclonal antibody supports investigations into neuronal RNA transport, dendritic mRNA localization, and synaptic plasticity. Beyond catalog antibodies, we provide validated antibody pairs, customized monoclonal and polyclonal development, bulk ancillary reagent supply, and dedicated sourcing for rare targets—helping you maintain project momentum with a single, reliable partner.