Anti-Stathmin 1 Polyclonal Antibody for WB, IHC-P, ELISA - P16949

Core Product Specifications and Parameters

Biological Background: Stathmin 1 Function and Localization

• Stathmin 1 (STMN1), also designated oncoprotein 18 (Op18) or phosphoprotein p19, is a ~17 kDa cytosolic phosphoprotein encoded by the STMN1 gene (Gene ID: 3925) that undergoes alternative splicing to generate multiple isoforms.
• It functions as a key regulator of microtubule dynamics by binding tubulin heterodimers, thereby preventing microtubule assembly and promoting catastrophe-driven disassembly.
• Phosphorylation at Ser-16 is implicated in axon formation during neurogenesis, linking stathmin to neuronal differentiation and synaptic plasticity (By similarity).
• Subcellularly, stathmin 1 is primarily cytoplasmic and associates with the cytoskeleton, consistent with its role in microtubule modulation.
• Expression is ubiquitous but markedly elevated in brain, spinal cord, and cerebellum, as well as in thymus, bone marrow, and testis; notably, it is overexpressed in acute leukemia cells compared to normal lymphocytes or other leukemia subtypes.
• Post-translational modifications including acetylation, methylation, and extensive phosphorylation regulate its activity and protein interactions, as indicated by direct protein sequencing and proteomic identifications.

Experimental Guidance and Technical Tips

• For Western blot (WB), expect a band at ~17 kDa for Stathmin 1; however, phosphorylation or isoform variations may cause slight shifts. Validate in positive control lysates such as leukemia cell lines (e.g., K562) or brain tissue.
• This rabbit polyclonal antibody was raised against an N-terminal peptide (aa 1-100) and detects human, mouse, and rat Stathmin 1. Cross-reactivity with other stathmin family members (e.g., STMN2-4) is plausible due to sequence homology; verify specificity with knockout or blocking peptide controls.
• For immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution; brain or tumor sections are recommended as positive tissues given the high expression in CNS and leukemia.
• In ELISA, use as either capture or detection antibody after pairwise testing; the linear epitope targeted lies within the N-terminal region, making it suitable for sandwich assays with a non-overlapping antibody clone.
• Ensure proper storage at -20°C or on ice packs during shipping to maintain stability; avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio accelerates your IVD development by providing integrated access to raw materials, from discovery to market. This Stathmin 1 antibody exemplifies our commitment to supplying high-quality reagents for microtubule dynamics and oncology research. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, ancillary reagents, and custom sourcing for niche targets — all backed by technical consultation to streamline your assay development pipeline.