Anti-Stathmin 1 Monoclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - P16949

Core Product Specifications and Parameters

Biological Background: Stathmin Function and Localization

• Stathmin (STMN1), also known as Oncoprotein 18 (Op18), Metablastin, or Leukemia-associated phosphoprotein p18, is a ubiquitously expressed 17 kDa cytoplasmic phosphoprotein belonging to the stathmin family.
• It functions as a critical regulator of microtubule dynamics, destabilizing microtubules by promoting their disassembly and preventing their assembly.
• Stathmin plays a pivotal role in cell cycle progression, mitosis, and differentiation, with its activity tightly controlled by phosphorylation at multiple serine residues (e.g., Ser-16, Ser-25, Ser-38, Ser-63).
• Phosphorylation at Ser-16 is implicated in axon formation during neurogenesis, while the protein also contributes to learned and innate fear responses (By similarity).
• Subcellularly, stathmin localizes to the cytoplasm and associates with the cytoskeleton, consistent with its microtubule-regulatory function.
• Tissue expression is broad but notably enriched in fetal and adult brain, spinal cord, cerebellum, thymus, bone marrow, testis, and fetal liver; its overexpression is a hallmark of acute leukemia and certain malignancies.
• Alternative splicing generates multiple isoforms, and the protein undergoes post-translational modifications including acetylation, methylation, and phosphorylation, impacting its stability and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 70–149 of human Stathmin 1, which includes critical phosphorylation and coiled-coil domains; this region may be suitable for detecting both phosphorylated and unmodified forms, but epitope accessibility might vary.
• For Western blotting, expect a band at ~17 kDa; use appropriate loading controls and consider phosphatase inhibitors to preserve phosphorylation states.
• In immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), strong cytoplasmic staining is anticipated; permeabilization is required, and co-staining with cytoskeletal markers (e.g., tubulin) can confirm localization.
• Given cross-reactivity with mouse and rat, validate the antibody in the relevant model system, as sequence homology in the immunogenic region may influence detection sensitivity.
• For ELISA applications, optimize coating antigen and antibody concentration empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For anti-Stathmin 1 antibodies and related reagents, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing to support oncology, neuroscience, and cell division research. Contact us to streamline your IVD development pipeline.