Anti-STAT6 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P42226

Core Product Specifications and Parameters

Biological Background: STAT6 Function and Localization

• STAT6 is a signal transducer and activator of transcription that mediates cellular responses to interleukin-4 (IL-4) and interleukin-3 (IL-3), playing a dual role in signal transduction and activation of transcription.
• The protein contains an SH2 domain, which is critical for recruitment to phosphorylated cytokine receptors, leading to Janus kinase (JAK)-mediated phosphorylation, dimerization, and nuclear translocation.
• Alternative splicing generates isoforms such as STAT6B and STAT6C, which may have distinct functions in modulating transcriptional activity.
• Post-translational modifications, including phosphorylation, acetylation, and ADP-ribosylation, regulate STAT6 stability, DNA-binding affinity, and transcriptional output.
• Under basal conditions, STAT6 is predominantly cytoplasmic, but cytokine stimulation triggers its relocalization to the nucleus to bind specific DNA response elements and activate target gene expression.
• Polymorphisms and mutations in STAT6 are linked to allergic and inflammatory diseases, underscoring its role in immune dysregulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 501–600, which may target a region accessible in both unphosphorylated and activated STAT6. Verify the antibody's ability to detect phosphorylated STAT6 if required.
• For Western blotting, a band at approximately 94 kDa is expected. Consider using IL-4 stimulated cell lysates to confirm activation-dependent signals.
• In immunofluorescence/ICC, STAT6 will appear diffuse in the cytoplasm in resting cells and concentrated in the nucleus upon cytokine treatment; include appropriate controls.
• For ELISA, use the antibody as a capture or detection reagent after validating specificity and sensitivity in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers by providing a comprehensive range of IVD raw materials. For studies on IL-4/STAT6 signaling and transcriptional regulation, we offer validated antibody pairs, optimized rabbit polyclonal antibodies like this STAT6 antibody, and bulk ancillary reagents. Whether you need routine supply or rare target sourcing, our one-stop platform bridges the gap from concept to clinic.