Anti-STAT5B Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P51692

Core Product Specifications and Parameters

Biological Background: STAT5B Function and Localization

• STAT5B functions as a signal transducer and transcription activator, mediating cellular responses to cytokines, hormones, and growth factors.
  Related references: PMID:29844444, PMID:9188471
• It binds to the gamma-activated sequence (GAS) elements in DNA, activating prolactin (PRL)-induced transcription.
• STAT5B positively regulates hematopoietic and erythroid differentiation.
• It participates in the oncostatin-M signaling pathway via the type II OSM receptor complex (OSMR/IL6ST), contributing to gene expression changes.
  Related references: PMID:9188471
• The protein shuttles between the cytoplasm and nucleus; upon activation, it translocates to the nucleus to exert transcriptional control.
• STAT5B contains an SH2 domain and is regulated by phosphorylation, making it a phosphoprotein involved in key signal transduction cascades.
• Mutations in STAT5B are associated with disease variants, including dwarfism, underscoring its essential role in growth regulation.
• The molecular weight of STAT5B is approximately 90 kDa, consistent with its 89866 Da predicted mass.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 594-794) of human STAT5B, which includes part of the SH2 domain; this design may provide specificity for both unphosphorylated and phosphorylated forms, but validation against relevant phospho-mutants is recommended.
• For Western blotting, expect a band near 90 kDa for STAT5B; start with a dilution range (e.g., 1:500–1:2000) and adjust based on signal intensity and background in your sample type.
• In immunohistochemistry, nuclear staining is anticipated upon pathway activation; optimize antigen retrieval and permeabilization conditions to preserve epitope accessibility and subcellular localization.
• For ELISA applications, consider using this antibody as a capture or detection reagent with appropriate blocking strategies; cross-reactivity with mouse STAT5B broadens experimental flexibility.
• As a rabbit polyclonal antibody, lot-to-lot consistency should be verified, and it is advisable to include appropriate positive and negative controls in all assays.

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CamelBio offers diagnostic developers and researchers a streamlined, one-stop sourcing platform for IVD raw materials, including premium antibodies against transcription factors like STAT5B. Our supply capabilities cover validated polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare-target sourcing to accelerate your assay development from research to clinical use. Partner with CamelBio to secure high-quality, scalable reagents tailored to your signal transduction research needs.