Anti-STAT5A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P42229

Core Product Specifications and Parameters

Biological Background: STAT5A Function and Localization

• STAT5A (Signal transducer and activator of transcription 5A) is a transcription factor that mediates signal transduction and activation of transcription in response to cytokines and growth factors.
• It plays a key role in the regulation of gene expression, particularly in the control of milk protein expression during lactation.
• STAT5A binds to the GAS element in gene promoters, activating transcription of target genes such as those involved in cell proliferation, differentiation, and survival.
• The protein localizes to the cytoplasm in its inactive state and translocates to the nucleus upon activation by phosphorylation.
• STAT5A is post-translationally modified by phosphorylation and ubiquitin-like conjugation, which regulate its activity and stability.
• Alternative splicing of STAT5A produces multiple isoforms, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 635-794 of human STAT5A, a region within the C-terminal transactivation domain. This design may help detect full-length STAT5A and certain isoforms.
• For Western blot (WB), a band around 91 kDa is expected (predicted molecular weight ~90.6 kDa). Optimize antibody dilution and blocking conditions for your specific sample.
• For immunofluorescence/immunocytochemistry (IF/ICC), permeabilization may be necessary to detect nuclear localization. Validate in cells known to express STAT5A.
• For ELISA, consider pairing with a matched detection antibody for sandwich assays. Validate in the relevant sample matrix.
• Cross-reactivity with mouse and rat STAT5A is indicated; however, verify specificity in your experimental model.

CamelBio: Your One-Stop Sourcing Bridge

As a leading IVD raw material supplier, CamelBio offers a one-stop sourcing solution for diagnostic manufacturers and research labs. This STAT5A antibody, along with a broad portfolio of validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents, supports your signal transduction and transcription research needs. Our team also specializes in sourcing rare-target raw materials to accelerate your assay development from concept to clinic.