Anti-STAT4 Rabbit Polyclonal Antibody for WB - Q14765

Core Product Specifications and Parameters

Biological Background: STAT4 Function and Localization

• STAT4 is a transcription factor predominantly expressed in hematopoietic cells, where it regulates cellular growth, differentiation, and immune responses.
  Related references:
  PMID:10961885
  PMID:37256972
  PMID:8943379

• Upon IL12 binding to its receptor IL12RB2, STAT4 is recruited, becomes tyrosine phosphorylated, homodimerizes, and translocates to the nucleus to activate IL12-responsive genes.
  Related references:
  PMID:10415122
  PMID:7638186

• STAT4 is a critical driver of T-helper 1 (Th1) cell differentiation and interferon-gamma (IFN-γ) production, essential for cell-mediated immunity.
  Related references:
  PMID:12213961
  PMID:35614130

• It also participates in neutrophil functions, including chemotaxis and the formation of neutrophil extracellular traps.

• In addition to IL12, STAT4 can be phosphorylated and activated by IFN-γ via JAK1/TYK2 and by interleukins such as IL23, IL2, and IL35.
  Related references:
  PMID:11114383
  PMID:34508746

• In response to IFN-α/β signaling, STAT4 acts as a transcriptional repressor, suppressing IL5 and IL13 mRNA expression during T-cell receptor activation.
  Related references:
  PMID:26990433

• STAT4 interacts with JUN to form a complex that binds AP-1-related sequences in the IFN-γ promoter, facilitating transcription.

• Post-translational modifications include acetylation and phosphorylation; the protein contains an SH2 domain and is associated with systemic lupus erythematosus (SLE). STAT4 dynamically localizes to both the cytoplasm and nucleus.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 569–748) of human STAT4, encompassing part of the SH2 domain and the transcriptional activation domain. This region exhibits high homology among human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blotting (WB), a band of approximately 86 kDa is expected. Use appropriate positive controls such as peripheral blood mononuclear cells or lymphoid tissues.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), both nuclear and cytoplasmic staining patterns may be observed, depending on cellular activation status. Consider including isotype controls and, if necessary, blocking peptides to confirm specificity.
• When performing ELISA, validate the antibody pair and optimize coating and detection conditions in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, providing manufacturers and labs with end-to-end support from concept to clinic. For immune response and T-cell signaling research, we supply validated STAT4 antibodies alongside optimized antibody pairs, recombinant proteins, and ancillary reagents in bulk. Our sourcing network helps you secure rare-target raw materials and complements your development workflow with technical consultation, ensuring a streamlined path from assay design to diagnostic production.