Anti-STAT4 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q14765

Core Product Specifications and Parameters

Biological Background: STAT4 Function and Localization

• STAT4 is a transcriptional regulator primarily expressed in hematopoietic cells, playing a critical role in cellular growth, differentiation, and immune responses. Related references: PMID:10961885, PMID:37256972, PMID:8943379
• It is essential for T-helper 1 (Th1) cell differentiation and the production of interferon-gamma (IFN-γ). Related references: PMID:12213961, PMID:35614130
• STAT4 participates in multiple neutrophil functions, including chemotaxis and the formation of neutrophil extracellular traps.
• Upon interleukin-12 (IL12) binding to its receptor IL12RB2, STAT4 interacts with the intracellular domain, becomes tyrosine-phosphorylated, homodimerizes, and translocates to the nucleus to regulate IL12-responsive genes. Related references: PMID:10415122, PMID:7638186
• STAT4 can also be phosphorylated and activated by IFN-gamma via JAK1 and TYK2, and by interleukins IL23, IL2, and IL35. Related references: PMID:11114383, PMID:34508746
• Transcriptional activation of the IFN-gamma gene is mediated by interaction with JUN, forming a complex at the AP-1-related promoter sequence.
• In response to IFN-alpha/beta signaling, STAT4 acts as a transcriptional repressor, suppressing IL5 and IL13 mRNA during T-cell receptor (TCR) activation. Related references: PMID:26990433
• STAT4 localizes to both the cytoplasm and nucleus, and its activity is regulated by post-translational modifications including acetylation and phosphorylation; it contains an SH2 domain and is associated with systemic lupus erythematosus.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide spanning amino acids 649–748 of human STAT4 (NP_003142.1), located in the C-terminal region. This design is suitable for detecting full-length STAT4 protein.
• For Western blotting, a band at approximately 86 kDa is expected. Verify specificity by using appropriate positive and negative controls.
• For immunofluorescence/immunocytochemistry, optimize fixation and permeabilization conditions for your cell type to ensure proper antigen accessibility.
• For ELISA applications, consider pairing with a suitable detection antibody and validate performance with recombinant STAT4 or cell lysates.
• Cross-reactivity with mouse and rat STAT4 has been observed, but sequence alignment is recommended to confirm epitope conservation in your species of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, bridging the gap from concept to clinic. For STAT4 research in immune function and inflammatory diseases, we provide ready-to-use validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare target raw-material procurement. Let us streamline your diagnostic development workflow.