Anti-STAT3 Rabbit Polyclonal Antibody for WB and ELISA - P40763

Core Product Specifications and Parameters

Biological Background: STAT3 Function and Localization

• STAT3 (Signal transducer and activator of transcription 3), also known as acute-phase response factor, is a transcription factor that mediates cellular responses to various cytokines and growth factors, including interleukins, KITLG/SCF, and LEP. Once activated, it recruits coactivators such as NCOA1 or MED1 to target gene promoters. Related references: PMID:10688651, PMID:11294841, PMID:12359225, PMID:15653507, PMID:16285960, PMID:17344214, PMID:12873986
• Upon activation of IL6ST/gp130 signaling by IL6, STAT3 binds to IL6-responsive elements in acute-phase protein gene promoters, and it is also activated by IL31 through IL31RA. Related references: PMID:12359225, PMID:15194700
• STAT3 serves as a critical regulator of inflammatory responses by controlling the differentiation of naive CD4+ T-cells into T-helper Th17 or regulatory T-cells (Treg), with acetylation promoting its transcriptional activity and differentiation, while deacetylation and oxidation by LOXL3 inhibit it. Related references: PMID:28065600, PMID:28262505
• It regulates cell cycle progression from G1 to S phase by inducing CCND1 expression and mediates LEP effects on body energy homeostasis, lactation, and apoptosis via BIRC5 transactivation. Related references: PMID:17344214, PMID:18242580
• Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity, and it plays a crucial role in basal pancreatic beta cell functions, including regulation of insulin secretion. Related references: PMID:23084476
• STAT3 is involved in oncostatin-M-mediated signaling through both type I (LIFR/IL6ST) and type II (OSMR/IL6ST) receptor complexes. Related references: PMID:9188471
• STAT3 dynamically shuttles between the cytoplasm and nucleus and is broadly expressed in tissues such as heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas, as well as in naive CD4+ T cells and T-helper Th17, Th1, Th2 subsets. Related references: PMID:31899195

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 130–770 of human STAT3, covering the majority of the protein, which may allow detection of multiple isoforms. Validate specificity in the relevant sample system.
• For Western blot (WB) applications, consider using reducing conditions and anticipate a band near 88 kDa; note that phosphorylation may cause slight mobility shifts.
• For ELISA, test various capture/detection conditions and confirm cross-reactivity with mouse and rat STAT3 if needed.
• As with any polyclonal antibody, lot-to-lot consistency should be verified for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with integrated access to IVD raw materials, technical services, and consulting—from concept to clinic. Our STAT3 antibody supports investigations into cytokine signaling, inflammatory diseases, and cancer biology. Beyond this product, we offer validated antibody pairs, bulk ancillary reagents, and rare target sourcing to streamline your assay development and production.