Anti-STAT3 Rabbit Monoclonal Antibody for WB and ELISA - P40763

Core Product Specifications and Parameters

Biological Background: STAT3 Function and Localization

• Signal transducer and activator of transcription that mediates cellular responses to interleukins (e.g., IL6, IL31), KITLG/SCF, LEP, and other growth factors. Related references: PMID:10688651 PMID:11294841 PMID:12359225
• Upon activation of IL6ST/gp130 by IL6, binds to IL6-responsive elements in promoters of acute-phase protein genes, and also mediates signaling through FGFRs (FGFR1-4). Related references: PMID:12873986 PMID:12359225
• Regulates inflammatory responses by controlling differentiation of naive CD4+ T-cells into T-helper Th17 or regulatory T-cells (Treg); acetylation promotes transcription activity, while deacetylation and oxidation by LOXL3 inhibit differentiation. Related references: PMID:28065600 PMID:28262505
• Involved in cell cycle progression from G1 to S phase by inducing expression of CCND1, and mediates LEP effects on melanocortin production and energy homeostasis. Related references: PMID:17344214
• Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity; also plays a role in basal beta cell functions including insulin secretion. Related references: PMID:23084476
• Participates in oncostatin-M signaling through both type I (LIFR/IL6ST) and type II (OSMR/IL6ST) receptor complexes. Related references: PMID:9188471
• Subcellular localization: shuttles between cytoplasm and nucleus. Tissue expression: heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, and in naive CD4+ T cells as well as Th17, Th1, and Th2 cells. Related references: PMID:31899195
• Post-translational modifications include phosphorylation, acetylation, methylation, and ubiquitination, which regulate its activity, DNA binding, and protein interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 600-700) of human STAT3. Consider that this region may be subject to post-translational modifications that could affect antibody binding; use appropriate phosphatase or deacetylase inhibitors if necessary.
• For Western blot, use standard sample preparation and include a phosphatase inhibitor cocktail to preserve phosphorylation states. A primary antibody dilution in the range of 1:500-1:2000 is commonly used for similar antibodies, but individual optimization is recommended.
• For ELISA, validate the antibody with recombinant STAT3 protein or the immunizing peptide to ensure specificity. The antibody may bind both human and mouse STAT3; confirm cross-reactivity in your assay system.
• Note that the predicted molecular weight is 88 kDa; however, due to phosphorylation and other modifications, the observed band may shift or appear as multiple bands. Include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For STAT3-related projects, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can source rare targets. Whether you're developing assays for JAK/STAT signaling, inflammation, or cancer biomarkers, CamelBio streamlines your raw-material procurement from discovery to production.