Anti-STAT2 Polyclonal Antibody for WB, IHC-P, ELISA - P52630

Core Product Specifications and Parameters

Biological Background: STAT2 Function and Localization

• STAT2 (Signal transducer and activator of transcription 2) is a critical transcription factor that mediates cellular responses to type I interferons (IFN-alpha and IFN-beta).
• Upon type I IFN stimulation, STAT2 undergoes tyrosine phosphorylation, heterodimerizes with STAT1, and associates with IRF9 to form the ISGF3 complex, which translocates to the nucleus and binds ISRE elements to drive transcription of interferon-stimulated genes (ISGs) (PubMed:9020188, 23391734).
  Related references:
  PMID:9020188
  PMID:23391734
• In addition to its activating role, STAT2 exerts negative feedback control on type I IFN signaling by recruiting the deubiquitinase USP18 to the IFNAR2 receptor subunit, thereby dampening the response (PubMed:28165510).
  Related references:
  PMID:28165510
• STAT2 also functions as a regulator of mitochondrial fission by modulating the phosphorylation of dynamin-related protein 1 (DNM1L) at Ser-616 and Ser-637 (PubMed:23391734, 26122121).
  Related references:
  PMID:23391734
  PMID:26122121
• The protein localizes to both the cytoplasm and the nucleus, shuttling in response to activation signals.
• STAT2 belongs to the STAT family of transcription factors and contains a conserved SH2 domain essential for its interaction with phosphorylated receptors and dimerization.
• It is subject to multiple post-translational modifications, including phosphorylation, and is implicated in antiviral defense, as highlighted by its role in host-virus interactions and disease variants.

Experimental Guidance and Technical Tips

• The immunogen (recombinant fragment encompassing amino acids 550–706) targets the C-terminal region of human STAT2, which includes part of the SH2 domain and linker domain; the resulting polyclonal antibody may recognize multiple isoforms and splice variants.
• For Western blotting, a band near 98 kDa is expected; treatment of cells with type I interferons (e.g., IFN-alpha) may enhance STAT2 expression and phosphorylation, providing a useful positive control.
• In IHC-P, optimization of antigen retrieval and antibody dilution is recommended; appropriate positive and negative tissue controls should be included.
• This antibody cross-reacts with mouse and rat STAT2, enabling its use in cross-species studies; however, validation in the relevant experimental system is advised.
• For ELISA applications, consider pairing with a monoclonal capture antibody to achieve a sensitive sandwich assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this STAT2 polyclonal antibody as part of a comprehensive portfolio of IVD raw materials designed for infectious disease and immune diagnostics. We support diagnostic manufacturers with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets, helping you move seamlessly from concept to clinic.