Anti-STAT1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P42224

Core Product Specifications and Parameters

Biological Background: STAT1 Function and Localization

• STAT1 is a signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokines, and growth factors. Related references: PMID:12764129 PMID:12855578 PMID:15322115
• Upon type I IFN stimulation, STAT1 is tyrosine-phosphorylated and forms the ISGF3 complex with STAT2 and IRF9, which translocates to the nucleus and binds ISRE to activate interferon-stimulated genes. Related references: PMID:28753426 PMID:35568036
• In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated, homodimerizes to form GAF, and binds GAS elements to drive antiviral gene expression. Related references: PMID:8156998 PMID:26479788
• STAT1 also mediates signaling from KITLG/SCF, FGFRs, and TLR4 pathways, indicating broad involvement in immune and cellular responses. Related references: PMID:15526160 PMID:19088846 PMID:32209697
• Alternative splicing produces STAT1 alpha (p91) and STAT1 beta (p84) isoforms, which differ in their C-terminal transactivation domains.
• Subcellular localization is primarily cytoplasmic, with nuclear translocation upon activation.
• Post-translational modifications include tyrosine and serine phosphorylation, acetylation, ADP-ribosylation, methylation, and ubiquitination, regulating its activity.
• STAT1 plays a role in food tolerance in the small intestine by associating with Gasdermin-D p13 and promoting CIITA transcription for Tr1 cell induction.

Experimental Guidance and Technical Tips

• The immunogen (residues 513–712) lies in the C-terminal region of human STAT1, which includes the SH2 domain and activation site; this design may yield antibodies recognizing both alpha and beta isoforms.
• For WB, IHC-P, and ELISA, consider optimal antibody dilutions by testing in the relevant sample system, as reactivity and background can vary.
• Cross-reactivity with mouse and rat is reported; however, it is advisable to validate performance in each species and application experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-STAT1 antibody supports research into interferon signaling and immune regulation, a critical area for infectious disease and oncology diagnostic development. Our comprehensive portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development.