Anti-STAT1 Rabbit Monoclonal Antibody for WB, IP, ELISA - P42224

Core Product Specifications and Parameters

Biological Background: STAT1 Function and Localization

• STAT1 exists as two isoforms, alpha (p91) and beta (p84), generated by alternative splicing.

• Acts as a signal transducer and transcription activator mediating cellular responses to interferons (IFNs), cytokine KITLG/SCF, and other growth factors. Related references: PMID:12764129, PMID:12855578, PMID:15322115

• Following type I IFN stimulation, STAT1 is tyrosine-phosphorylated, dimerizes with STAT2 and IRF9 to form the ISGF3 complex, which binds ISRE elements and activates antiviral interferon-stimulated genes. Related references: PMID:28753426, PMID:35568036

• In response to type II IFN (IFN-gamma), STAT1 is phosphorylated and homodimerizes to form the GAF complex, which translocates to the nucleus and binds GAS sequences to drive transcription of target genes. Related references: PMID:8156998, PMID:26479788

• STAT1 becomes activated by KITLG/SCF-KIT signaling and may mediate cellular responses to activated FGFR1, FGFR2, FGFR3, and FGFR4. Related references: PMID:15526160, PMID:19088846

• Upon bacterial LPS-induced TLR4 endocytosis, IKBKB phosphorylates STAT1 at Thr-749, promoting its binding to specific promoters and activation of ARID5A and IL12B transcription. Related references: PMID:32209697

• STAT1 shuttles between the cytoplasm and nucleus; its activation leads to nuclear accumulation where it regulates gene expression.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100), potentially detecting both alpha and beta STAT1 isoforms; consider verifying band patterns in your samples.
• For Western blot, include phosphatase inhibitors if analyzing endogenous STAT1 to preserve phosphorylation states.
• Validate antibody specificity in your experimental system (e.g., using STAT1 knockout lysates or siRNA knockdown) before drawing quantitative conclusions.
• The antibody is suitable for immunoprecipitation; optimize antibody-to-lysate ratios and incubation conditions for your target protein abundance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research labs with comprehensive access to IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target sourcing. Our STAT1 antibody supports studies on interferon signaling, antiviral immunity, and STAT1-related diseases. Partner with CamelBio to streamline your raw-material procurement and accelerate your project timelines.