Anti-STARD3NL Polyclonal Antibody for WB, IHC-P, ELISA - O95772

Core Product Specifications and Parameters

Biological Background: STARD3NL Function and Localization

• STARD3NL (STARD3 N-terminal-like protein), also known as MENTHO (MLN64 N-terminal domain homolog), is encoded by the STARD3NL gene in humans.
• It functions as a tethering protein that creates membrane contact sites between the endoplasmic reticulum and late endosomes by interacting with VAPA and VAPB on the ER.
• Related references: PMID:24105263
• STARD3NL is an integral membrane protein localized to the late endosome membrane, with a single predicted transmembrane helix.
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomic studies.
• Alternative initiation produces different isoforms from the same gene.
• The protein belongs to the STARD3 family, which is involved in lipid transport and organelle contact site formation.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 185–234) lies in a conserved region; the antibody may recognize multiple isoforms if the epitope is shared. Validate specificity using knockout or overexpression models.
• For Western blot (WB), the predicted molecular weight is ~27 kDa; consider using positive control lysates from human, mouse, or rat tissues.
• In immunohistochemistry (IHC-P), antigen retrieval conditions should be optimized as the epitope might be masked. Use appropriate permeabilization for late endosome staining.
• ELISA performance can vary; consider checkerboard titrations to determine optimal coating and detection antibody concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and research laboratories by supplying validated raw materials for assay development. This STARD3NL antibody belongs to our membrane trafficking portfolio, with immediate availability for WB, IHC-P, and ELISA applications. Beyond this product, we offer one-stop access to matched antibody pairs, recombinant proteins, and ancillary reagents—backed by technical consultation to streamline your transition from benchtop to validated immunoassay.