Anti-StAR (STARD1) Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - P49675

Core Product Specifications and Parameters

Biological Background: StAR Function and Localization

• StAR (Steroidogenic acute regulatory protein), also known as STARD1 (START domain-containing protein 1), is a mitochondrial protein crucial for steroid hormone biosynthesis.
• It facilitates the rate-limiting transfer of cholesterol from the outer to the inner mitochondrial membrane, where cholesterol is converted to pregnenolone by the cytochrome P450 side-chain cleavage enzyme.
• Belongs to the START (StAR-related lipid transfer) domain family, which includes proteins involved in lipid transport and metabolism.
• Localized to the mitochondrion, with reported presence at the outer membrane and matrix; contains a mitochondrial transit peptide that directs its import.
• Highly expressed in steroidogenic tissues such as gonads (testis, ovary), adrenal cortex, and kidney.
• Mutations in the STAR gene are associated with congenital adrenal hyperplasia, a disorder of steroidogenesis, highlighting its essential role in hormone production.
• The protein undergoes phosphorylation, which may regulate its activity and import into mitochondria.
• The predicted molecular weight is approximately 31.9 kDa, though the antibody detects a band around 32 kDa in Western blot assays.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 64-285 of human StAR (NP_000340.2). This region covers a large part of the protein, potentially providing broad epitope recognition. For Western blot, a band around 32 kDa is expected; consider using whole-cell lysates from steroidogenic tissues or cell lines as positive controls.
• Validated for Western blot, immunofluorescence (IF/ICC and IF-P), and ELISA. For IF, mitochondrial staining patterns should be anticipated; co-staining with mitochondrial markers may assist with subcellular localization studies.
• Cross-reacts with human, mouse, and rat StAR based on supplier data; however, researchers should validate species cross-reactivity in their own experimental systems, as reactivity may vary with post-translational modifications or sample preparation.
• Since StAR is a mitochondrial protein, ensure that lysis and staining protocols are optimized for organelle preservation when assessing localization or function.
• For ELISA, the use of recombinant protein or peptide antigens may help confirm specificity and sensitivity; consider testing multiple antibody dilutions to establish optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop platform for IVD raw materials, offering high-quality polyclonal and monoclonal antibodies like this anti-StAR antibody. We support researchers and diagnostic developers with validated antibody pairs, optimized immunoreagents, and bulk ancillary materials for steroidogenesis and lipid transport studies. For rare or custom targets, our sourcing network helps bridge gaps from concept to clinic.