Anti-StAR (STARD1) Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P49675

Core Product Specifications and Parameters

Biological Background: StAR Function and Localization

• StAR (Steroidogenic acute regulatory protein), also known as STARD1, is a mitochondrial protein encoded by the STAR gene (GeneID: 6770) in humans.
• It plays a central role in steroid hormone biosynthesis by facilitating the transfer of cholesterol from the outer to the inner mitochondrial membrane, where cholesterol is converted to pregnenolone.
• This rate-limiting step is essential for the production of all steroid hormones, including glucocorticoids, mineralocorticoids, and sex steroids.
• The protein localizes to the mitochondrion outer membrane and matrix, consistent with its function in cholesterol trafficking.
• StAR is predominantly expressed in steroidogenic tissues, such as the gonads, adrenal cortex, and kidney.
• Post-translational modifications include phosphorylation, and mutations in STAR are associated with congenital adrenal hyperplasia (CAH), a disorder of steroidogenesis.
• Classified under lipid transport and cholesterol metabolism, StAR contains a START domain that binds cholesterol and other lipids.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 100-200 of human StAR (NP_000340.2). For Western blot, expect a band at approximately 32 kDa.
• This antibody has been validated for use in WB, IF-P, IHC-P, and ELISA. For each application, optimize working dilutions using your specific experimental conditions.
• Confirm cross-reactivity in mouse and rat samples, as indicated; if extending to other species, validate specificity via sequence alignment.
• For functional studies, consider using steroidogenic cell lines (e.g., mouse Leydig tumor cells or human adrenocortical cells) to monitor StAR expression changes under hormone stimulation.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop IVD raw-material sourcing partner, CamelBio supports diagnostic manufacturers, labs, and research institutes with a comprehensive portfolio of high-quality antibodies, antigen pairs, and ancillary reagents. The StAR Rabbit Monoclonal Antibody is offered alongside validated antibody pairs and optimized monoclonal/polyclonal antibodies for steroidogenesis and adrenal-related research areas. Whether you need bulk reagents or custom sourcing for rare targets, CamelBio streamlines your supply chain from concept to clinic.