Anti-StAR Rabbit Polyclonal Antibody for WB, IP, ELISA - P49675

Core Product Specifications and Parameters

Biological Background: StAR Function and Localization

• StAR (Steroidogenic Acute Regulatory protein), also known as STARD1, is a mitochondrial protein crucial for steroid hormone biosynthesis.
• Its primary function is to mediate the transfer of cholesterol from the outer to the inner mitochondrial membrane, the rate-limiting step in steroidogenesis, leading to pregnenolone production.
• Subcellular localization is predominantly at the mitochondrion outer membrane and matrix, facilitating substrate access.
• Tissue expression is high in steroidogenic tissues: gonads (testes, ovaries), adrenal cortex, and kidney.
• StAR is a phosphoprotein, and phosphorylation at specific residues may regulate its activity and mitochondrial import.
• Mutations in the STAR gene cause congenital adrenal hyperplasia, underscoring its essential role in adrenal and gonadal steroidogenesis.
• The protein contains a START domain, which binds lipids and cholesterol, mediating transport.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 64-285) covers most of the START domain; thus, the antibody may recognize both full-length StAR and possibly a processed form. Validate specificity in your model system.
• For Western blot (WB), expect a band around 32 kDa. As a polyclonal antibody, minor additional bands may appear; use appropriate positive controls (e.g., mouse testis or adrenal tissue lysates). Optimize blocking and antibody dilution.
• For immunoprecipitation (IP), pre-clear lysates and use a standard rabbit IgG control to reduce non-specific binding.
• For ELISA, assess direct or capture format depending on your assay design; consider testing against recombinant StAR or peptide to confirm reactivity.
• Cross-reactivity is reported for human and mouse; for other species, check sequence alignment of the immunogen region, as the antibody may cross-react with conserved domains.
• Store and handle according to the shipping condition (ice bag); avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to provide validated IVD raw materials including this anti-StAR polyclonal antibody, tailored for steroidogenesis studies. We offer bulk quantities, ancillary reagents (detection antibodies, antigens), and technical consulting to streamline your assay development. Contact us for a complete sourcing solution from concept to clinic.