Anti-STAMBPL1 Polyclonal Antibody for WB and ELISA - Q96FJ0

Core Product Specifications and Parameters

Biological Background: STAMBPL1 Function and Localization

• STAMBPL1 (AMSH-like protease, STAM-binding protein-like 1) is a zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains, playing a critical role in ubiquitin signaling. Related references: PMID:18758443 PMID:35114100
• It acts as a positive regulator of the TORC1 signaling pathway by mediating 'Lys-63'-linked deubiquitination of SESN2, thereby inhibiting SESN2 interaction with the GATOR2 complex. Related references: PMID:35114100
• The enzyme does not cleave 'Lys-48'-linked polyubiquitin chains, demonstrating linkage-specific deubiquitinase activity. Related references: PMID:18758443
• STAMBPL1 is ubiquitously expressed across tissues, suggesting a housekeeping role in cellular ubiquitin homeostasis.
• The protein is subject to post-translational modifications including phosphorylation and acetylation, and contains metal-binding sites essential for its catalytic activity (UniProt keywords: Phosphoprotein, Acetylation, Metal-binding, Zinc).
• Alternative splicing of the STAMBPL1 gene produces multiple isoforms, which may have distinct regulatory functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 120-250 of human STAMBPL1 (NP_065850.1), a region that may contribute to epitope specificity. Consider verifying the antibody's recognition of this domain in your experimental system.
• For Western blot, the predicted molecular weight of STAMBPL1 is approximately 50 kDa (UniProt: 49.8 kDa). Due to potential post-translational modifications or alternative splicing, additional bands may be observed; optimize loading and blocking conditions as needed.
• This polyclonal antibody is suitable for ELISA and WB. Cross-reactivity with mouse and rat STAMBPL1 has been reported, enabling its use in rodent model studies. Always validate the antibody in the relevant species and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio delivers comprehensive IVD raw-material solutions, connecting diagnostic manufacturers and researchers with validated antibody reagents and rare-target sourcing. This anti-STAMBPL1 polyclonal antibody supports studies in ubiquitin signaling and TORC1 pathway dysregulation, with complementing services including custom antibody development, bulk ancillary reagents, and technical consulting to accelerate your project from concept to clinic.