Anti-STAMBP Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - O95630

Core Product Specifications and Parameters

Biological Background: STAMBP Function and Localization

• Protein identity: STAMBP, also known as AMSH (Associated molecule with the SH3 domain of STAM) and MICCAP, is an endosome-associated ubiquitin isopeptidase encoded by the STAMBP gene.
• Lys-63-specific deubiquitinase: It is a zinc metalloprotease that specifically hydrolyzes 'Lys-63'-linked polyubiquitin chains, with no activity toward 'Lys-48' linkages (PubMed:15314065, PubMed:23542699, PubMed:34425109).
  Related references:
  PMID:15314065
  PMID:23542699
  PMID:34425109
• IL-2/GM-CSF signaling: Participates in signal transduction downstream of IL-2 and GM-CSF, contributing to cell growth and MYC induction (PubMed:10383417).
  Related references:
  PMID:10383417
• BMP signaling potentiation: Potentiates bone morphogenetic protein (BMP) signaling by antagonizing the inhibitory actions of SMAD6 and SMAD7 (PubMed:11483516).
  Related references:
  PMID:11483516
• Endocytic sorting: Plays a key role in ubiquitin-dependent sorting of cell surface receptors to lysosomes; its endosomal localization is essential for efficient EGFR degradation but not for receptor internalization (PubMed:15314065, PubMed:17261583).
  Related references:
  PMID:15314065
  PMID:17261583
• Negative regulation of signaling pathways: Involved in negative regulation of the PI3K-AKT-mTOR and RAS-MAP signaling cascades (PubMed:23542699).
  Related references:
  PMID:23542699
• Subcellular localization: Localizes to the nucleus, cytoplasm, membrane, and early endosomes.
• Tissue distribution: Ubiquitously expressed across human tissues.
• Post-translational modifications: Is a phosphoprotein and subject to alternative splicing, yielding multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (amino acids 100–270) of human STAMBP, which shares high sequence homology with mouse and rat orthologs. Cross-reactivity with mouse and rat is expected; validate in the relevant sample system.
• For Western blotting, the predicted molecular weight is approximately 48 kDa. Consider the potential impact of phosphorylation or other post-translational modifications on electrophoretic mobility, and use appropriate positive and negative controls.
• When performing immunohistochemistry or immunocytochemistry, antigen retrieval conditions and blocking steps should be optimized. Given STAMBP’s localization to multiple compartments, include subcellular markers in your analysis.
• In immunoprecipitation assays, maintain native protein–protein interactions; STAMBP interacts with components of the endosomal sorting machinery, which may require mild lysis conditions.
• This polyclonal antibody may be used in ELISA; however, direct detection limits should be determined empirically for the target antigen and assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, CROs, and academic labs with a comprehensive selection of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. The STAMBP polyclonal antibody is designed for research into ubiquitin-mediated signaling, endosomal trafficking, and growth factor pathways. Through our integrated sourcing platform, we also support procurement of bulk raw materials and assist in identifying rare-target reagents—streamlining your workflow from early discovery to clinical application.