Anti-STAM2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O75886

Core Product Specifications and Parameters

Biological Background: STAM2 Function and Localization

• STAM2 (Signal transducing adapter molecule 2), also known as Hrs-binding protein (Hbp), is encoded by the STAM2 gene and exists in multiple isoforms due to alternative splicing.
• It functions in intracellular signal transduction mediated by cytokines and growth factors, specifically playing a role in IL-2 and GM-CSF stimulated signaling leading to DNA synthesis and MYC induction.
• STAM2 is implicated in T-cell development, contributing to proper immune cell maturation and function.
• The protein is a component of the ESCRT-0 complex with HGS, which is essential for the down-regulation of receptor tyrosine kinases via the multivesicular body (MVB) pathway; the complex binds ubiquitinated receptors and sorts them for lysosomal degradation.
• Subcellularly, STAM2 localizes to the cytoplasm and early endosome membrane, consistent with its role in endosomal sorting.
• It contains an SH3 domain and is a phosphoprotein, subject to post-translational modifications that may regulate its activity.
• STAM2 is ubiquitously expressed across tissues, indicating broad involvement in cellular signaling and trafficking processes.

Experimental Guidance and Technical Tips

• For Western blot (WB), the expected molecular weight is approximately 58 kDa; consider using a suitable loading control and confirm specificity with knockout or knockdown samples.
• In immunofluorescence/immunocytochemistry (IF/ICC), expect staining in the cytoplasm and possibly punctuate early endosome structures; use appropriate fixation and permeabilization protocols.
• For ELISA, validated detection of STAM2 in human, mouse, and rat samples is indicated; include appropriate standards and negative controls.
• As this is a polyclonal antibody generated against a recombinant fragment (aa 376-525), it may recognize multiple epitopes; lot-to-lot consistency should be checked for critical assays.
• Cross-reactivity with human, mouse, and rat is reported; for other species, sequence homology should be examined before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing solution for STAM2 antibodies and related raw materials, supporting research in cytokine signaling, receptor trafficking, and T-cell development. Our portfolio includes validated polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom development services to accelerate your IVD assay development from concept to clinic. Partner with us for reliable, high-quality raw materials that meet the demands of diagnostic manufacturers and research laboratories.