Anti-STAM Rabbit Polyclonal Antibody for WB and ELISA - Q92783

Core Product Specifications and Parameters

Biological Background: STAM Function and Localization

• STAM (Signal transducing adapter molecule 1), also known as STAM1 or STAM-1, is a ubiquitously expressed adapter protein that participates in intracellular signal transduction downstream of cytokines and growth factors.
• Upon IL-2 and GM-CSF stimulation, STAM contributes to signaling cascades that promote DNA synthesis and MYC induction, highlighting its role in cell proliferation and survival.
• STAM is a component of the ESCRT-0 complex (together with HGS), which recognizes ubiquitinated receptors and sorts them into multivesicular bodies (MVBs) for lysosomal degradation, thereby down-regulating receptor tyrosine kinases.
• The ESCRT-0 complex functions as a sorting machinery on the cytoplasmic face of early endosome membranes, where STAM is predominantly localized.
• STAM has been implicated in T-cell development, consistent with its expression in immune tissues and its involvement in cytokine signaling.
• In a microbial infection context, STAM plays a critical role in Dengue virus entry, underscoring its relevance beyond endogenous cellular processes.
• STAM is a phosphoprotein and contains an SH3 domain, which mediates protein-protein interactions; it can undergo isopeptide bond formation and ubiquitin conjugation, further diversifying its regulatory functions.
• Alternative splicing of STAM produces multiple isoforms, potentially contributing to tissue-specific or signal-specific adaptations.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 120–210 of human STAM, a region that may be conserved across mouse and rat, consistent with the reported cross-reactivity; consider sequence alignment for confirmation.
• For Western blot, a band around 59 kDa is expected; validate specificity in lysates from human, mouse, or rat cell lines expressing STAM.
• When performing ELISA, use recombinant STAM protein or peptides spanning the immunogen region as coating antigens; titrate the antibody to optimize sensitivity.
• As this is a polyclonal antibody, lot-to-lot variability may occur; it is advisable to include positive and negative controls and determine the optimal working dilution for each application.
• Consider testing the antibody in immunoprecipitation or immunofluorescence, as STAM localizes to early endosomes; however, published validation may be necessary for these applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, streamlining your access to validated antibody pairs, optimized polyclonal antibodies like anti-STAM, and bulk ancillary reagents. Our expertise spans from signal transduction research to infectious disease targets such as Dengue virus entry, supporting diagnostic manufacturers, labs, and research institutes from concept to clinic. Contact us to explore tailored sourcing solutions that accelerate the development of your immunoassays and research applications.