Anti-ST8SIA2 Polyclonal Antibody for WB and ELISA - Q92186

Core Product Specifications and Parameters

Biological Background: ST8SIA2 Function and Localization

• ST8SIA2 (alpha-2,8-sialyltransferase 8B, also known as SIAT8-B, STX, ST8SiaII) catalyzes the transfer of sialic acid residues via α-2,8-linkages onto terminal sialic acids of N-linked glycans, playing a central role in polysialic acid (PSA) synthesis on substrates such as NCAM1, FETUB, and AHSG (PubMed:10766765, PubMed:11744634, PubMed:9054414, PubMed:7559389). Related references: PMID:10766765 PMID:11744634 PMID:9054414 PMID:7559389
• The enzyme lacks sialic acid-polymerase activity, indicating a specialized role in the initiation or elongation of PSA chains rather than de novo synthesis (By similarity).
• In the hippocampus, ST8SIA2-mediated polysialylation of NCAM1 promotes neurite outgrowth and may support oligodendrocyte differentiation and myelin/axon integrity (By similarity) (PubMed:9054414). Related references: PMID:9054414
• Subcellularly, the protein is primarily located at the Golgi apparatus membrane, but it can also be secreted and detected at the cell membrane.
• Tissue expression is highest in fetal brain, kidney, and heart, with lower abundance in adult heart and thymus.
• The glycoprotein contains a signal-anchor transmembrane domain and disulfide bonds, features typical of Golgi-resident glycosyltransferases.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 24–160), which may enable detection of both full-length and some truncated isoforms; verify band patterns in relevant sample systems.
• For Western blot, a band around 42 kDa is expected under reducing conditions; optimize primary antibody dilution ranging from 1:500 to 1:2000 based on target abundance.
• Cross-reactivity with mouse and rat suggests high sequence homology; validate specificity using knockout controls or siRNA knockdown where possible.
• ELISA protocols may require direct coating of the immunogen or capture format; consider checkerboard titrations to identify optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering this ST8SIA2 antibody alongside a curated portfolio for glycosylation and neurobiology research. We supply validated antibody pairs and polyclonal/monoclonal antibodies in bulk, supported by technical consultation to streamline your assay development from concept to clinical application.