Anti-ST8SIA1 Polyclonal Antibody for WB, ELISA - Q92185

Core Product Specifications and Parameters

Biological Background: ST8SIA1 Function and Localization

• ST8SIA1 (Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase), also known as GD3 synthase, is a glycosyltransferase that adds sialic acid in alpha-2,8-linkage to GM3 to form ganglioside GD3, a key step in ganglioside biosynthesis (PubMed:18348864, PubMed:22885356, PubMed:7937974). Related references: PMID:18348864 PMID:22885356 PMID:7937974
• It can further extend GD3 to GT3 and utilizes substrates like GM1b, GD1a, and GT1b to synthesize complex gangliosides GD1c, GT1a, and GQ1b (PubMed:8631981, PubMed:8706663). Related references: PMID:8631981 PMID:8706663
• The protein is anchored in the Golgi apparatus membrane with a type II topology, characteristic of sialyltransferases, and possesses a signal-anchor sequence and a transmembrane helix.
• Alternative splicing generates multiple isoforms; the canonical isoform has a molecular weight of approximately 40.5 kDa (UniProt: 40519 Da), while the observed immunogen-based weight is 41 kDa.
• ST8SIA1 is strongly expressed in melanoma cell lines, adult and fetal brain, and to a lesser extent in adult and fetal lung, consistent with its role in neural and cancer-associated ganglioside patterns.
• It contains disulfide bonds and N-linked glycosylation sites, which are critical for proper folding and enzymatic activity in the Golgi.
• In breast cancer cells, it can synthesize unusual tetra- and pentasialylated lactosylceramide derivatives GQ3 and GP3, highlighting its role in cancer-associated glycosylation (PubMed:22885356). Related references: PMID:22885356

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 49–270, a region likely lacking the transmembrane domain, suggesting antibody recognition of the soluble catalytic domain. For WB, use reduced denaturing conditions and a positive control (e.g., melanoma cell lysate) to confirm bands near 41 kDa.
• Due to the polyclonal nature, multiple isoforms or post-translational modifications may be detected. Consider treating samples with PNGase F to assess glycosylation-dependent shifts.
• Cross-reactivity with mouse ST8SIA1 is specified, enabling studies in mouse models. Validate species-specific reactivity in the relevant sample system before large-scale experiments.
• For ELISA, the antibody can be used as a capture or detection reagent; titrate optimally against recombinant ST8SIA1 or cell-derived ganglioside extracts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and researchers to provide one-stop access to IVD raw materials, including this anti-ST8SIA1 polyclonal antibody. As a specialist in ganglioside and sialyltransferase targets, CamelBio supports your workflow with validated antibody pairs, monoclonal/polyclonal options, and bulk ancillary reagents. From concept to clinic, we help secure rare-target raw materials, streamlining your development in cancer and neuroscience applications.