Anti-ST6GAL1/CD75 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, FC, ELISA - P15907

Core Product Specifications and Parameters

Biological Background: ST6GAL1/CD75 Function and Localization

• ST6GAL1, also known as CD75, ST6N, SIAT1, and ST6GalI, is a beta-galactoside alpha-2,6-sialyltransferase that catalyzes the transfer of sialic acid to galactose-containing acceptor substrates.
• In B lymphocytes, this enzyme generates neuraminidase-sensitive cell-surface differentiation antigens, including CDw75, HB-6, and CD76 (PubMed:1730763). Related references: PMID:1730763
• ST6GAL1 sialylates complex-type N-glycans on the Fc region of immunoglobulins, conferring anti-inflammatory effector functions to IgG.
• The enzyme preferentially monosialylates the alpha(1->3) mannose antenna of Fc glycoforms, with subsequent disialylation occurring at a much slower rate.
• Subcellularly, ST6GAL1 localizes to the Golgi stack membrane, consistent with its role in glycan processing, and can also be secreted.
• Alternative splicing produces multiple isoforms, with this antibody recognizing forms at approximately 20 kDa and 47 kDa, as indicated by the product data.
• Post-translational modifications reported for ST6GAL1 include glycosylation, disulfide bond formation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 44-406 of human ST6GAL1, covering nearly the entire extracellular domain; this broad region may allow detection of multiple isoforms.
• For Western blotting, consider the predicted molecular weights (20 kDa and 47 kDa) and validate detection in your sample system, optimizing protein loading and antibody concentration as needed.
• In immunohistochemistry (IHC-P) and immunocytochemistry (IF/ICC), ensure appropriate fixation and permeabilization protocols to preserve antigenicity.
• Flow cytometry (FC) and ELISA applications suggest the antibody recognizes native protein; include proper isotype controls and validation with known positive and negative samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with comprehensive access to IVD raw materials, supporting every stage from concept to clinic. This anti-ST6GAL1/CD75 monoclonal antibody exemplifies our portfolio of high-quality immunological reagents for glycosylation and immunology research. Beyond individual antibodies, we supply validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, ensuring your development pipeline stays on track.