Anti-ST6GAL1 Polyclonal Antibody for WB, IHC-P, ELISA - P15907

Core Product Specifications and Parameters

Biological Background: ST6GAL1 Function and Localization

• ST6GAL1 encodes beta-galactoside alpha-2,6-sialyltransferase 1 (ST6Gal I, SIAT1, CDw75), a type II membrane protein resident in the Golgi apparatus.
• Catalyzes the transfer of sialic acid from CMP-sialic acid to terminal galactose residues on N-glycans, producing α2,6-linked sialylated glycoconjugates.
• In B lymphocytes, ST6GAL1 generates the CDw75, HB-6 and CD76 cell-surface differentiation antigens.
  Related references:
  PMID:1730763
• Mediates sialylation of the Fc region of IgG antibodies, preferentially on the α1,3 mannose antenna, conferring anti-inflammatory properties.
• Located in the Golgi stack membrane, with a cleaved soluble form also secreted.
• Undergoes alternative splicing, producing multiple isoforms; annotated as a glycoprotein and phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal luminal domain (aa 27-200), which may detect multiple ST6GAL1 isoforms. In Western blot, confirm the band size (~47 kDa) under reducing conditions.
• For IHC-P, antigen retrieval protocols (e.g., heat-induced epitope retrieval) and appropriate tissue controls are recommended to optimize staining specificity.
• In ELISA, use recombinant ST6GAL1 protein or peptide as coating antigen; cross-reactivity with mouse and rat orthologs enables comparative studies.
• Avoid using samples with high endogenous IgG if detecting IgG-sialylated targets, as this may cause interference.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material sourcing with end-to-end support from concept to clinic. This anti-ST6GAL1 polyclonal antibody serves glycosylation research, immune cell biology, and antibody engineering applications. Contact us for bulk orders, validated antibody pairs, and customized reagent solutions tailored to your diagnostic or research workflows.