Anti-ST3GAL5 Polyclonal Antibody for WB, ELISA - Q9UNP4

Core Product Specifications and Parameters

Biological Background: ST3GAL5 Function and Localization

• The ST3GAL5 gene encodes the enzyme lactosylceramide alpha-2,3-sialyltransferase, commonly known as GM3 synthase, ST3Gal V, or sialyltransferase 9 (SIAT9).
• It catalyzes the transfer of N-acetylneuraminic acid (NeuAc) from CMP-NeuAc to the terminal galactose of lactosylceramide (LacCer) to form ganglioside GM3, a critical step in glycosphingolipid biosynthesis that regulates multiple cellular processes including proliferation, differentiation, and apoptosis. Related references: PMID:16934889 PMID:9822625
• The enzyme preferentially uses LacCer but can also sialylate other glycolipids such as galactosylceramide (GalCer), asialo-GM2 (GA2), and asialo-GM1 (GA1) with lower efficiency.
• ST3GAL5 mRNA is ubiquitously expressed, with highest levels in the brain (cerebral cortex, temporal lobe, putamen), skeletal muscle, placenta, and testis.
• Mutations in ST3GAL5 are linked to severe neurological disorders, including infantile-onset epileptic encephalopathy and intellectual disability, underscoring its essential role in brain development.
• The protein is localized to the Golgi apparatus membrane, contains a signal-anchor transmembrane domain, forms disulfide bonds, and undergoes alternative splicing to generate multiple isoforms.

Experimental Guidance and Technical Tips

• For Western blot, use denaturing conditions; the predicted molecular weight is approximately 48 kDa, but monitor for possible dimerization or post-translational modifications.
• Validate the antibody specificity with appropriate positive and negative controls (e.g., cell lines with known expression, siRNA knockdown, or knockout lysates) in your experimental system.
• For ELISA, titrate the antibody to determine optimal working concentration and assess cross-reactivity with related sialyltransferases if necessary.
• When working with tissue samples, consider that ST3GAL5 expression may vary; include loading controls and confirm expression levels in your target tissue.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, contract research organizations, and clinical laboratories with one-stop access to IVD raw materials and technical services. To support ganglioside and ST3GAL5-related research and assay development, we offer validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Partner with us to streamline your path from concept to clinic.