Anti-ST3GAL4 Rabbit Polyclonal Antibody (WB, ELISA) - Q11206

Core Product Specifications and Parameters

Biological Background: ST3GAL4 Function and Localization

• ST3GAL4 encodes a beta-galactoside alpha-2,3-sialyltransferase that catalyzes the transfer of sialic acid from CMP-Neu5Ac to Galbeta-(1->3)-GalNAc and Galbeta-(1->4)-GlcNAc-terminated glycoconjugates, mediating terminal sialylation of glycoproteins and glycolipids.
  Related references:
  PMID:8288606
  PMID:8611500
• Plays a key role in hemostasis by sialylating plasma von Willebrand factor, thereby preventing its recognition by asialoglycoprotein receptors and subsequent clearance, and also regulates ASGPR-mediated platelet clearance.
• Participates in the biosynthesis of sialyl Lewis X epitopes on both O- and N-glycans, which serve as ligands for selectins, and is essential for selectin-mediated leukocyte rolling and adhesion during extravasation.
  Related references:
  PMID:25498912
• In glycosphingolipid metabolism, sialylates GM1 and GA1 gangliosides to form GD1a and GM1b, respectively, and synthesizes ganglioside LM1, a major structural component of peripheral nerve myelin.
  Related references:
  PMID:8288606
  PMID:8611500
• Localizes primarily to the Golgi stack membrane and can also be secreted.
• Highly expressed in adult placenta, heart, and kidney.
• Features alternative splicing, disulfide bonds, and glycosylation; belongs to the glycosyltransferase 29 family.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 234-333 of human ST3GAL4 (isoform NP_001241686.1). This region may be conserved across isoforms; consider sequence alignment to verify specificity.
• Validated for Western blot (WB) and ELISA applications. For WB, a band around 36-38 kDa is expected; adjust electrophoresis conditions to resolve potential isoforms.
• Cross-reactivity with mouse has been confirmed; validate in the relevant sample system for your experimental design.
• As a polyclonal antibody, batch-to-batch variability may occur; include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. In sialyltransferase and glycosylation research, we support your development with high-quality reagents and technical expertise from concept to clinic.