Anti-ST3GAL4 Polyclonal Antibody for WB, ELISA - Q11206

Core Product Specifications and Parameters

Biological Background: ST3GAL4 Function and Localization

• ST3GAL4 encodes CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 4 (alpha 2,3-sialyltransferase IV), also known as SAT-3, ST-4, ST3GalIV, and STZ, a key member of the sialyltransferase family.
• It catalyzes the transfer of sialic acid (Neu5Ac) from CMP-Neu5Ac onto Galbeta-(1->3)-GalNAc- and Galbeta-(1->4)-GlcNAc-terminated glycoconjugates through an alpha2-3 linkage, mediating terminal sialylation of glycoproteins and glycolipids (PubMed:8288606, PubMed:8611500, PubMed:37632720). Related references: PMID:8288606, PMID:8611500, PMID:37632720
• Plays a major role in hemostasis by sialylating plasma von Willebrand factor (VWF), which prevents its recognition by asialoglycoprotein receptors (ASGPR) and subsequent clearance; also regulates ASGPR-mediated platelet clearance.
• Essential for selectin-mediated leukocyte rolling and adhesion: participates in the biosynthesis of sialyl Lewis X epitopes on O- and N-glycans, recognized by SELE, SELP, and SELL during extravasation (PubMed:25498912). Related references: PMID:25498912
• In glycosphingolipid biosynthesis, sialylates GM1 and GA1 gangliosides to form GD1a and GM1b, respectively (PubMed:8288606); also metabolizes brain c-series ganglioside GT1c to GQ1c and synthesizes ganglioside LM1, a major structural component of peripheral nerve myelin (PubMed:8611500). Related references: PMID:8288606, PMID:8611500
• Localizes predominantly to the Golgi apparatus and Golgi stack membrane; can also be secreted into the extracellular space.
• Highly expressed in adult placenta, heart, and kidney.

Experimental Guidance and Technical Tips

• For Western blot (WB), expect a band at approximately 38 kDa; however, N-glycosylation may cause a higher apparent molecular weight. Validate using relevant cell or tissue lysates.
• The antibody is polyclonal and cross-reacts with mouse ST3GAL4; confirm reactivity in mouse samples using appropriate positive and negative controls.
• In ELISA, consider using the recombinant immunogen (amino acids 27–267) as coating antigen; optimize antibody dilutions and assay conditions for your specific format.
• Always include species-specific controls and validate the antibody in your experimental system to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio of antibodies, antigens, and ancillary reagents tailored for glycobiology and hemostasis research. With this anti-ST3GAL4 polyclonal antibody, you benefit from our validated product line and the ability to source matched antibody pairs, bulk polyclonal antibodies, and rare target reagents for diagnostic development. Partner with CamelBio to streamline your supply chain from concept to clinical application.