Anti-ST3GAL1 Rabbit Polyclonal Antibody for WB and ELISA - Q11201

Core Product Specifications and Parameters

Biological Background: ST3GAL1 Function and Localization

• ST3GAL1 (CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1), also known as SIAT4A or ST3Gal I, is a member of the glycosyltransferase family involved in glycoprotein and glycolipid sialylation.
• It catalyzes the transfer of sialic acid (Neu5Ac) from CMP-Neu5Ac to Galbeta-(1->3)-GalNAc-terminated glycoconjugates via an alpha2-3 linkage, a key step in terminal sialylation. Related references: PMID:31784620 PMID:8027041 PMID:31719620
• ST3GAL1 specifically adds sialic acid to core 1 O-glycans (Galbeta-(1->3)-GalNAc-O-Ser/Thr), the major structure of mucin-type O-glycans, thereby modifying glycoproteins such as SPN/CD43 and PTPRC/CD45 on T cells.
• Through sialylation of T cell glycoproteins, ST3GAL1 plays a homeostatic role in regulating CD8-positive T cell numbers by preventing premature apoptosis in thymic T cells and controlling survival of memory T cells in secondary lymphoid organs.
• It also acts on gangliosides, converting GM1a to GD1a, GA1 to GM1b, and GD1b to GT1b. Related references: PMID:8027041
• The enzyme localizes to the Golgi apparatus, specifically the Golgi stack membrane and trans-Golgi network membrane; a soluble secreted form has also been identified.
• Expression of ST3GAL1 is widespread, with highest levels detected in lung, liver, skeletal muscle, kidney, pancreas, spleen, and placenta.
• The protein is a transmembrane glycoprotein with a signal-anchor, containing disulfide bonds and a short cytoplasmic tail.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider using a dilution range of 1:500 to 1:2000 and validate with positive control lysates from tissues known to express ST3GAL1, such as lung or liver.
• For ELISA, the recombinant immunogen (amino acids 35-135) may serve as a suitable positive control; optimize coating and detection conditions empirically.
• As the antibody shows cross-reactivity with mouse and rat, it can be employed for orthologous studies, but always confirm specificity in the relevant species context.
• Include appropriate negative controls (e.g., knockout cell lines or peptide blocking) to verify specificity, especially given the polyclonal nature of the antibody.
• Note that ST3GAL1 is a transmembrane Golgi protein; for WB, a membrane-enriched fraction may improve signal detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers in glycobiology and sialylation-related studies. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets like ST3GAL1. Our platform streamlines procurement, helping you accelerate from concept to clinic.