Anti-SSX4 Polyclonal Antibody for WB, ELISA - O60224

Core Product Specifications and Parameters

Biological Background: SSX4 Function and Localization

• SSX4, also known as Cancer/testis antigen 5.4 (CT5.4), is a member of the SSX family of proteins encoded by the SSX4 gene (including isoforms SSX4A and SSX4B).
• It may function as a modulator of transcription, implicating it in the regulation of gene expression.
• Classified as a cancer/testis antigen, SSX4 is normally expressed in testis but aberrantly expressed in various cancers, making it a potential immunotherapeutic target.
• The protein undergoes alternative splicing, producing different isoforms that may have distinct functional roles.
• SSX4 has been identified in proteomics studies, supporting its expression at the protein level.
• Molecular weight of the canonical isoform is approximately 22 kDa.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider using whole cell lysates from human or mouse cell lines expressing SSX4. Validate antibody specificity with positive and negative controls.
• In ELISA applications, the immunogen corresponding to amino acids 70-188 may be used as a coating antigen or standard. Optimal dilution should be determined experimentally.
• Given the predicted molecular weight of 22 kDa, ensure appropriate gel percentage and transfer conditions for low-molecular-weight proteins.
• As SSX4 expression may be restricted, verify target presence in your sample system before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering a streamlined supply chain from concept to clinic. This SSX4 polyclonal antibody exemplifies our commitment to providing validated reagents for transcription and cancer research. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can even source rare-target raw materials to meet your diagnostic development needs.