Anti-SSX2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q16385

Core Product Specifications and Parameters

Biological Background: SSX2 Function and Localization

• Protein SSX2, also known as cancer/testis antigen 5.2 (CT5.2), synovial sarcoma X breakpoint 2, and tumor antigen HOM-MEL-40, is encoded by the SSX2 gene (GeneID: 6757) with an alternatively spliced isoform SSX2B.
• SSX2 is suggested to act as a modulator of transcription, potentially playing a role in gene regulation.
• It localizes to the nucleus, consistent with its putative role in transcriptional regulation.
• Expression is highly restricted, with high levels in the testis and low levels in the thyroid; it is absent in most normal somatic tissues but aberrantly expressed in some sarcoma cell lines.
• Classified as a proto-oncogene and a phosphoprotein, SSX2 is implicated in chromosomal rearrangements associated with synovial sarcoma.
• Belonging to the SSX family of cancer/testis antigens, SSX2 is frequently investigated as a target in cancer immunotherapy.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–188) of human SSX2; consider this when designing detection or blocking strategies.
• For WB, the predicted protein weight is 22 kDa; validate band specificity using positive controls such as testis lysates or SSX2-expressing cell lines.
• In IF/ICC, confirm nuclear localization using appropriate fixation and permeabilization protocols.
• Cross-reactivity with mouse and rat has been observed; validate in the relevant sample system if working with these species.
• For ELISA, titrate the antibody and include appropriate negative controls to ensure signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials. This Anti-SSX2 polyclonal antibody supports oncology and transcriptional regulation research, complementing our offerings such as validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. We streamline your supply chain, enabling seamless progression from concept to clinic.