Anti-SSTR1 Rabbit Polyclonal Antibody for WB, ELISA - P30872

Core Product Specifications and Parameters

Biological Background: SSTR1 Function and Localization

• SSTR1 is a high-affinity receptor for somatostatin-14 over somatostatin-28, belonging to the G protein-coupled receptor (GPCR) superfamily.
• It mediates signaling through pertussis toxin-sensitive G proteins to inhibit adenylyl cyclase activity, reducing intracellular cAMP levels.
• Additionally, SSTR1 stimulates phosphotyrosine phosphatase activity and activates the Na(+)/H(+) exchanger via pertussis toxin-insensitive G proteins.
• The receptor is localized to the cell membrane and features seven transmembrane domains, along with post-translational modifications such as glycosylation and palmitoylation.
• SSTR1 is expressed in fetal kidney and liver, as well as in adult pancreas, brain, lung, jejunum, and stomach.
• The protein has a predicted molecular weight of approximately 42.7 kDa (UniProt), with observed bands around 43 kDa in experimental settings.
• As a disulfide bond-containing glycoprotein and lipoprotein, SSTR1 participates in diverse signaling pathways relevant to neuroendocrine and gastrointestinal research.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular region (aa 1-56), which is a target for antibody binding in native and denaturing conditions, supporting use in WB.
• For WB, consider using a whole-cell or membrane fraction lysate from human or rat tissues known to express SSTR1 (e.g., pancreas, brain, or stomach) to validate specificity.
• In ELISA, the recombinant immunogen or full-length SSTR1 protein can serve as a coating antigen; cross-reactivity with rat SSTR1 suggests utility in rat model studies.
• Optimization of primary antibody dilution and blocking conditions is recommended, as non-specific binding may occur due to the polyclonal nature of the antibody.
• Confirm detection of the ~43 kDa band under reducing and non-reducing conditions to assess potential disulfide bond-dependent epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents. For GPCR-focused projects like SSTR1 research, we offer one-stop sourcing from concept to clinic, streamlining your diagnostic development workflow. Our expertise covers rare targets and bulk supply, ensuring consistent quality for your assay needs.