Anti-SSRP1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q08945

Core Product Specifications and Parameters

Biological Background: SSRP1 Function and Localization

• SSRP1 (FACT80, T160) is a subunit of the Facilitates Chromatin Transcription (FACT) complex, a general chromatin factor that reorganizes nucleosomes to facilitate DNA-templated processes including transcription elongation, DNA replication, and DNA repair.
• The FACT complex functions as a histone chaperone: during transcription, it promotes the transient dissociation of one H2A-H2B dimer from the nucleosome to allow RNA polymerase II passage, then reassembles the nucleosome afterward.
• SSRP1 binds specifically to double-stranded DNA and shows low-affinity binding to cisplatin-modified DNA; this interaction may potentiate cisplatin-induced cell death by blocking replication and repair of damaged DNA.
• It acts as a transcriptional coactivator for p63/TP63 and may contribute to p53/TP53 phosphorylation at Ser-392 via an association with casein kinase II (CK2).
• Encoded by the SSRP1 gene on chromosome 11, the protein is 81 kDa and is also known as FACT80 and T160.
• Subcellular localization is predominantly nuclear, including the nucleolus, and it is associated with chromosomes.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin conjugation (isopeptide bond formation), as identified by proteomics and direct protein sequencing.
• The protein is widely expressed and involved in host-virus interactions, underscoring its central role in chromatin biology.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1–300 of human SSRP1; this N-terminal region may be conserved across isoforms, so assess isoform specificity if required.
• For Western blot (WB), the predicted molecular weight is approximately 81 kDa; include appropriate positive control lysates (e.g., HeLa, HEK293) and optimize loading and transfer conditions.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), a nuclear and nucleolar staining pattern is anticipated; validate in relevant tissue types using proper fixation and antigen retrieval methods.
• ELISA can be employed for quantitative detection, but standard curves and validation with recombinant protein or known samples are recommended for accurate quantitation.
• Cross-reactivity has been confirmed for human; reactivity with other species has not been determined, so validate in the relevant sample system before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement with one-stop access to validated antibodies, recombinant proteins, and ancillary reagents. This anti-SSRP1 antibody supports chromatin biology, transcription regulation, and DNA repair research. Whether you need optimized polyclonal or monoclonal antibodies, bulk reagents, or rare target sourcing, CamelBio bridges your diagnostic development from concept to clinic.