Anti-SSR3 Rabbit Polyclonal Antibody for WB and ELISA - Q9UNL2

Core Product Specifications and Parameters

Biological Background: SSR3 Function and Localization

• SSR3 encodes the signal sequence receptor subunit gamma, also known as translocon-associated protein subunit gamma (TRAPG), a component of the TRAP complex.
• The TRAP complex is involved in calcium binding at the endoplasmic reticulum (ER) membrane, regulating the retention of ER-resident proteins.
• SSR3 is an integral membrane protein with transmembrane domains, localizing to the ER membrane.
• Alternative splicing of SSR3 generates multiple transcript variants, though the functional significance may vary.
• Post-translational modifications include acetylation and phosphorylation, which may modulate its activity or interactions.
• The SSR3 protein has a predicted molecular weight of approximately 21 kDa, consistent with its size as a small transmembrane subunit.
• Keywords associated with SSR3 include 3D-structure, acetylation, alternative splicing, endoplasmic reticulum, membrane, phosphoprotein, proteomics identification, reference proteome, and transmembrane helix, highlighting its structural and functional characteristics.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 70–145 of human SSR3; consider that this region may be conserved across species, supporting the observed cross-reactivity with mouse and rat.
• For Western blotting, optimize antibody dilution and validate with positive and negative controls using lysates from human, mouse, or rat cell lines.
• For ELISA, test the antibody in indirect or sandwich formats with recombinant SSR3 protein or cell lysates; pairing with a suitable detection antibody is recommended.
• Store the antibody appropriately upon receipt, as it is shipped on ice to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering everything from optimized antibodies and recombinant proteins to technical services for assay development. For research on the translocon complex and ER protein retention, we supply high-quality SSR3 antibodies and can assist with sourcing rare targets or developing custom immune-reagents. With comprehensive support from concept to clinic, CamelBio accelerates your diagnostic and research workflows.