Anti-SSR2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P43308

Core Product Specifications and Parameters

Biological Background: SSR2 Function and Localization

• SSR2, also known as translocon-associated protein subunit beta (TRAP-beta) or signal sequence receptor subunit beta (SSR-beta), is a subunit of the TRAP complex, a heterotetrameric assembly located in the endoplasmic reticulum (ER) membrane.
• The TRAP complex, of which SSR2 is an integral part, functions to bind calcium to the ER membrane, thereby playing a crucial role in the regulation of ER resident protein retention.
• As an ER membrane protein, SSR2 contains a signal sequence and a transmembrane helix, anchoring it within the lipid bilayer and orienting its functional domains toward the ER lumen or cytoplasm.
• SSR2 is a glycoprotein, with post-translational modifications that may influence its stability, localization, or interactions within the ER environment.
• The human SSR2 gene encodes a protein with a predicted molecular weight of approximately 20 kDa, consistent with its detection in western blot analyses.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to amino acids 18-145 of human SSR2, which includes the luminal domain. This region is expected to be exposed in native conformations, making it suitable for applications such as IF/ICC and ELISA that may require detection of native epitopes.
• For western blotting, a band around 20 kDa is anticipated. Optimize sample preparation and gel percentage to achieve clear separation in this low molecular weight range.
• In immunohistochemistry (IHC-P), consider antigen retrieval methods such as heat-induced epitope retrieval, as the target is a membrane protein that may be masked by cross-linking.
• For immunofluorescence (IF/ICC), permeabilization is required to allow antibody access to the intracellular ER membrane compartment. Use appropriate detergents (e.g., Triton X-100) and include controls for background staining.
• This polyclonal antibody has been raised in rabbit and shows cross-reactivity with human, mouse, and rat, enabling versatile use across common model organisms. Validate in the relevant sample system to confirm specificity and signal strength.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents for bulk procurement. For studies on ER function and protein retention, this SSR2 antibody is part of our offering, and we can assist with sourcing rare-target reagents or tailored technical consulting to accelerate your assay development from concept to clinic.