Anti-SSCA1/SerpinB3 Polyclonal Antibody for WB, IF/ICC, ELISA - P29508

Core Product Specifications and Parameters

Biological Background: SSCA1/SerpinB3 Function and Localization

• Serpin B3 (SSCA1/SerpinB3), also known as squamous cell carcinoma antigen 1 (SCCA-1) or protein T4-A, belongs to the serpin superfamily of serine/cysteine protease inhibitors.
• It may act as a papain-like cysteine protease inhibitor, potentially modulating the host immune response against tumor cells.
• Serpin B3 functions as an inhibitor of UV-induced apoptosis by suppressing the activity of c-Jun NH2-terminal kinase (JNK1).
• The protein is predominantly localized in the cytoplasm.
• SSCA1/SerpinB3 is expressed in squamous cells and has been detected at the protein level in some hepatocellular carcinomas.
• Post-translational modifications include acetylation, and multiple isoforms are produced by alternative splicing.
• The three-dimensional structure of Serpin B3 has been determined, aiding structure-function studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 2-390 of human Serpin B3, covering nearly the full-length protein; this broad epitope coverage may enhance detection sensitivity across isoforms.
• For Western blot, a band of approximately 45 kDa is expected; validate in relevant sample systems such as squamous carcinoma cell lines or tissues.
• In immunofluorescence/immunocytochemistry, anticipate cytoplasmic staining; include appropriate controls and optimize fixation/permeabilization conditions.
• When using ELISA, consider pairing with a matched detection antibody and validate with recombinant Serpin B3 protein standards.
• Cross-reactivity with mouse allows application in mouse xenograft models, but confirm reactivity in the specific model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs to a comprehensive portfolio of IVD raw materials. This anti-SSCA1/SerpinB3 polyclonal antibody supports the development of assays targeting squamous cell carcinoma biomarkers and apoptosis-related pathways. From validated antibody pairs and bulk ancillary reagents to rare target sourcing, CamelBio streamlines procurement with technical consulting to accelerate your project from concept to clinic.